Background and Purpose: Soil remediation in contaminated soils is an important factor of environmental research. This research evaluated the effect of sepiolite, palygorskite, and TRIPLE SUPER PHOSPHATE (TSP) on decreasing the heavy metals uptake by wheat cultivars inoculated with Piriformospora indica (P. indica). Materials and Methods: Treatments included applying sepiolite, palygorskite (at the rate of 0% and 5% (W/W), and TSP (0% and 0. 5% W/W) in the Pb-and Zn-polluted soil under cultivation of 2 wheat cultivars (Mihan and Pishgam cultivars) that inoculated with P. indica fungus. Plants were harvested after 90 days, and Pb and Zn concentrations in soils and plants were measured using atomic absorption spectroscopy. Furthermore, the catalase (CAT) enzyme activity was also measured. Results: With applying 5% (W/W) sepiolite and palygorskite, the plant Pb concentration was significantly reduced by 11. 8%, while the plant Zn concentration increased by 13. 9%. Plant Zn and Pb concentrations considerably increased and decreased following plant inoculation. However, plant cultivars showed different results. Our results showed that the Pb and Zn concentrations were lower in Mihan than in the Pishgam wheat cultivar. In addition, increasing soil Pb availability caused a significant increase in CAT enzyme activity. Soil contamination with heavy metals had a negative impact on plant root colonization. Conclusion: The interaction of plant cultivars and P. indica significantly affected plant heavy metal uptake by plants. However, environmental studies have found that applying organic amendments like nan-clays or TSP can reduce the absorption of Pb by plants.