Vibrational spectroscopy is a complete tool for identifying and determining the structure of organic compounds, including drugs, food, polymer materials, explosives, etc. When talking about vibrational spectroscopy, usually the name of infrared method comes to mind, not Raman spectroscopy. In recent decades, there has been a development in the Raman method, especially in its instrumental part. These developments have led to its unique applications in various industries. This article deals with theory, instrumentation, sampling methods and applications of Raman spectroscopy.