EXTRACTION OF MORPHINE FROM HUMAN URINE USING LIQUID-LIQUID-EXTRACTION METHOD AND SOLID PHASE AND THEIR COMPARISON WITH SLIT SCANNING DENSITOMETRY

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THAGHAVI S.A.; NAZERI A.; FEKRI M.; SABZEVARI O.; AFSHAR M.

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Journal: Research in Medicine Year:2003 | Volume:27 | Issue:1 Page(s): 23-27

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Title

EXTRACTION OF MORPHINE FROM HUMAN URINE USING LIQUID-LIQUID-EXTRACTION METHOD AND SOLID PHASE AND THEIR COMPARISON WITH SLIT SCANNING DENSITOMETRY

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Keywords

MORPHINEQ3

LIQUID-LIQUID EXTRACTIONQ3

GAS SOLID PHASEQ4

Abstract

Background: There are various methods for MORPHINE extraction and detection, for this, Thin Layer Chromatography (TLC) is a useful tool. The most common detection technique of TLC plates is slit scanning densitometry. The aim of the present study was to compare the ability of a solid phase extraction (SPE) system with LIQUID-LIQUID EXTRACTION (LLE) for urinary detection of MORPHINE by a TLC scanner.Materials and methods: Ten urine samples were collected from opioid abusers and each sample was divided in 4; two fractions were extracted by the SPE system and the other two were extracted by LLE technique (Chloroform/2-Propanol, shaking and centrifugation). One sample of each fraction was hydrolysed. Finally, all samples were quantitatively analyzed by TLC-scanner.Results: Results have revealed that the area under curve (AUC) on hydrolyzed samples was higher than non hydrolyzed ones.Conclusion: Both techniques have showed better results for hydrolyzed samples. The recovery rate of SPE and LLE techniques were 94 and 80% respectively.

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DETECTION OF MORPHINE IN OPIOID ABUSERS HAIR BY GC/MS

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APA: Copy

THAGHAVI, S.A., NAZERI, A., FEKRI, M., SABZEVARI, O., & AFSHAR, M.. (2003). EXTRACTION OF MORPHINE FROM HUMAN URINE USING LIQUID-LIQUID-EXTRACTION METHOD AND SOLID PHASE AND THEIR COMPARISON WITH SLIT SCANNING DENSITOMETRY. PAJOUHESH DAR PEZESHKI, 27(1), 23-27. SID. https://sid.ir/paper/41951/en

Vancouver: Copy

THAGHAVI S.A., NAZERI A., FEKRI M., SABZEVARI O., AFSHAR M.. EXTRACTION OF MORPHINE FROM HUMAN URINE USING LIQUID-LIQUID-EXTRACTION METHOD AND SOLID PHASE AND THEIR COMPARISON WITH SLIT SCANNING DENSITOMETRY. PAJOUHESH DAR PEZESHKI[Internet]. 2003;27(1):23-27. Available from: https://sid.ir/paper/41951/en

IEEE: Copy

S.A. THAGHAVI, A. NAZERI, M. FEKRI, O. SABZEVARI, and M. AFSHAR, “EXTRACTION OF MORPHINE FROM HUMAN URINE USING LIQUID-LIQUID-EXTRACTION METHOD AND SOLID PHASE AND THEIR COMPARISON WITH SLIT SCANNING DENSITOMETRY,” PAJOUHESH DAR PEZESHKI, vol. 27, no. 1, pp. 23–27, 2003, [Online]. Available: https://sid.ir/paper/41951/en

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