Effect of Hypoxia Preconditioned Adipose-Derived Mesenchymal Stem Cell Conditioned Medium on Cerulein-Induced Acute Pancreatitis in Mice

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ABDOLMOHAMMADI Kamal; Mahmoudi Tayebeh; Nojehdehi Shahrzad; Tayebi Lobat; HASHEMI SEYED MAHMOUD; Noorbakhsh Farshid; ABDOLLAHI ALIREZA; SOLEIMANI MASOUD; NIKBIN BEHROUZ; NICKNAM MOHAMMAD HOSSEIN

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Journal: ADVANCED PHARMACEUTICAL BULLETIN Year:2020 | Volume:10 | Issue:2 Page(s): 297-306

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Title

Effect of Hypoxia Preconditioned Adipose-Derived Mesenchymal Stem Cell Conditioned Medium on Cerulein-Induced Acute Pancreatitis in Mice

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Keywords

Inflammation

Acute pancreatitis

Cerulein

Mesenchymal stem cell

Conditioned medium

Preconditioning

Abstract

Purpose: Acute pancreatitis (AP) is an inflammatory disorder distinguished by tissue injury and inflammation of the pancreas. Using paracrine potential of mesenchymal stem cells (MSCs) provides a useful clinical approach in treating inflammatory diseases. We investigated the therapeutic effects of adipose-derived MSC conditioned medium (CM) and hypoxia preconditioned adipose-derived MSC conditioned medium (HCM) in cerulein-induced AP in mice. Methods: AP was induced in C57BL/6 mice by intraperitoneal injection of cerulein (75 μ g/ kg/h × 7 times). One hour following the last injection of cerulein, mice were treated with intraperitoneal injection of CM and HCM (500 µ L/mice/30 min × 3 times). Twelve hours following the treatment, serum levels of amylase and lipase were measured. In addition, pancreas pathological changes, immunohistochemical examinations for evaluation of IL-6 expression and pancreatic myeloperoxidase (MPO) enzyme activity were analyzed. Results: The in vitro results of the morphological, differentiation and immunophenotyping analyses confirmed that hypoxia preconditioned MSCs (HP-MSCs) conserve MSCs characteristics after preconditioning. However, HP-MSCs significantly expressed high mRNA level of hypoxia inducible factor 1-α and higher level of total protein. The in vivo findings of the current study showed that CM and HCM significantly reduced the amylase & lipase activity, the severity of pancreas tissue injury and the expression of IL-6 and MPO enzyme activity compared with the AP group. However, no significant difference between CM and HCM groups was demonstrated. Conclusion: Use of CM and HCM can attenuate cerulein-induced AP and decrease inflammation in the pancreas tissue in AP mice.

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APA: Copy

ABDOLMOHAMMADI, Kamal, Mahmoudi, Tayebeh, Nojehdehi, Shahrzad, Tayebi, Lobat, Noorbakhsh, Farshid, ABDOLLAHI, ALIREZA, SOLEIMANI, MASOUD, NIKBIN, BEHROUZ, & NICKNAM, MOHAMMAD HOSSEIN. (2020). Effect of Hypoxia Preconditioned Adipose-Derived Mesenchymal Stem Cell Conditioned Medium on Cerulein-Induced Acute Pancreatitis in Mice. ADVANCED PHARMACEUTICAL BULLETIN, 10(2), 297-306. SID. https://sid.ir/paper/331913/en

Vancouver: Copy

ABDOLMOHAMMADI Kamal, Mahmoudi Tayebeh, Nojehdehi Shahrzad, Tayebi Lobat, Noorbakhsh Farshid, ABDOLLAHI ALIREZA, SOLEIMANI MASOUD, NIKBIN BEHROUZ, NICKNAM MOHAMMAD HOSSEIN. Effect of Hypoxia Preconditioned Adipose-Derived Mesenchymal Stem Cell Conditioned Medium on Cerulein-Induced Acute Pancreatitis in Mice. ADVANCED PHARMACEUTICAL BULLETIN[Internet]. 2020;10(2):297-306. Available from: https://sid.ir/paper/331913/en

IEEE: Copy

Kamal ABDOLMOHAMMADI, Tayebeh Mahmoudi, Shahrzad Nojehdehi, Lobat Tayebi, Farshid Noorbakhsh, ALIREZA ABDOLLAHI, MASOUD SOLEIMANI, BEHROUZ NIKBIN, and MOHAMMAD HOSSEIN NICKNAM, “Effect of Hypoxia Preconditioned Adipose-Derived Mesenchymal Stem Cell Conditioned Medium on Cerulein-Induced Acute Pancreatitis in Mice,” ADVANCED PHARMACEUTICAL BULLETIN, vol. 10, no. 2, pp. 297–306, 2020, [Online]. Available: https://sid.ir/paper/331913/en

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