Objective: Identification of toxigenic and nontoxigenic fungi agents isolated from feedstuffs, understudy.
Design: laboratory study.
Samples: Nineteen different samples of feedstuffs such as corn, oat, silage, concentrated feed, fat powder and etc.
Procedure: After processing of the samples, they were cultured (using inoculation & streak culture methods) on sabouraud glucose agar containing chloramphenicol, potato glucose agar,czapek-dox agar and rosebangal agar media under standard laboratory technical conditions.
Statistical analysis: Chi-square and t-test.
Results: The most frequent isolated fungi were: Aspergillus (63.2%), Penicillium (36.8%), Mucor (31.6%) and Cladosporium (26/3%), respectively. According to the colony counts, Fusarium colonies were the most abundantly isolated from silage and concentrated feeds (118 colonies).Among the isolated Aspergillus species, A.fumigatus (18.5%), A.flavus (35.9%), A.niger (25.9%) and A.sPP (29.6%) were identified. There was a significant defference between Aspergillus and the other fungi. These differences were significant, respect to suspected toxigenic and nontoxigenic species, as well. It means that toxigenic species were isolated more significantly than nontoxigenic agents.
Conclusion: With attention to presence of toxigenic fungi on the feeds, it should be considered to plan a program for identifying fungi(spacially toxigenic fungi )in different feedstuffs .After this step, in order to hygienic control of fungi on feeds, it is necessary to design some programs into the future.