Background & Aim: It is well established that the main cause of inflammatory diseases of dental pulps is dental caries, which in turn is induced by microorganisms. The presence of immunoglobulin within the inflamed pulp has been nidely investigated. It is suggested that immune responses could also participate in pathogenesis of pulpitis. The aim of this study was to determine the correlation between presence and concentration of pulpal IgG, IgA and IgM and various states of carious lesions.Material & method: For this purpose, a total of forty pulpal samples were evaluated. They were divided into four groups by clinical and radiographic examinations. Each group consisted of 10 samples of either healthy erupted teeth or carious lesions in enamel or carious lesions in outer half of dentin, or carious lesions in inner half of dentin. Samples were stored in explants cultures for 72 hours. SRID method was used to detect and quantitate IgG, IgA and IgM in supernatant fluids. Statistical analysis was made by Kruskal Wallis, Mann- Whitney U, Chi square and Fisher exact tests.Results: There was no IgA or IgM in all the samples. IgG was present only in 10% of the samples of caries in the inner half of the dentin eases with mean level of 96±287.9 mg/100 ml. There was no significant difference between different states of dental caries and the presence and/or concentration of IgG. Although the mean level and presence of IgG was higher in the samples with lesions in the inner half of the dentin.Conclusion: It could be suggested that probably humoral immune response has no important role in early inflammatory changes of dental pulps in reversible pulpitis.

Background: Zoonotic cutaneous leishmaniasis (ZCL) is an increasing public health problem in some endemic regions. Horseradish peroxidase (HRP) conjugated rabbit anti-Rhombomys opimus (R. opimus) Ig is needed for immunoblotting and ELISA tests used to explore the immune response of the rodents against the sand fly saliva. In this study, the production of HRP conjugated rabbit anti-R. opimus Ig was conducted for the first time.Methods: Rhombomys opimus Ig was purified from serum by protein G affinity chromatography column and injected into rabbit to produce anti-R. opimus Ig antibody. The titration of antibody against R. opimus Ig in rabbit serum was checked using indirect ELISA. Rabbit anti-R. opimus Ig was purified by Sepharose-4B- R. opimus Ig column. Reactivity of this antibody was assessed by indirect ELISA and was conjugated to HRP by periodate method.Results: Approximately 3.5 mg Ig was purified from 1 ml R. opimus serum using protein G affinity chromatography column. The molecular weight of purified R. opimus Ig was estimated about 150 kDa by SDS-PAGE. Nearly 2.3 mg rabbit anti-R. opimus Ig was purified from 1 ml immunized rabbit serum. The purified antibody was conjugated to HRP and the optimum titer of HRP conjugated rabbit anti-R. opimus Ig was determined as 1: 8000 using direct ELISA.Conclusion: HRP conjugated rabbit anti-Gerbil IgG has been produced by a few companies, but to our knowledge HRP conjugated rabbit anti-R. opimus Ig is not commercially available. Production of HRP conjugated rabbit anti- R. opimusIg is considerably helpful for immunological studies of R. opimus, the main reservoir host of ZCL in Iran as well as some other countries.

BACKGROUND AND OBJECTIVE: The defensive role of saliva is mainly played via immunoglobulins. Periodontal infections are the most common diseases in oral cavity and therefore defensive function of saliva against these infections are important. With regard to the effects of systemic diseases on defensive function of saliva, the objective of this study was to survey the relationship between gingivitis and salivary immunoglobulins in patients with thalassemia major. METHODS: This study was done on 55 children (between 5-12 years old) with thalassemia major who referred to thalassemia centre of Amirkola children hospital. Of these, 30 cases with gingivitis and 25 without gingivitis were chosen as case and control groups, respectively. Questionnaires were prepared and then evaluation of gingiva condition was done. Saliva samples were taken and examined by ELISA. FINDINGS: There was no a significant difference between the rate of IgA, IgG and IgM in two groups. Also, there was not relationship between severity of gingivitis and the rate of salivary immunoglobulins.CONCLUSION: T- cells affect on the activity of B cells. With regard to reduction of activity of T-cells lymphocyte (CD4) in thalassemia and the effect of these cells on promotion of B- lymphocyte function, B- cells can not response to gingivitis by synthesis of immunoglobulins.