Background and Aim: At present, there are various methods for performing diagnostic tests. Due to the fact that SALIVA is easily available compared to other body fluids, it can be collected from patients in a noninvasive way and there is no risk of cross-infection because of it, so its use has become more common. Also, the analysis of substances in SALIVA can be a reflection of a person's oral and systemic conditions. Despite its advantages as a diagnostic fluid, the lack of sufficient information about the methods of collecting and storing samples prevents the repeated use of SALIVA. In this article, it has been tried to collect the most standard methods of SALIVA collection, required conditions and supplies, and how to maintain, and provide them to users. Material and Methods: In this review article, data were collected by searching articles which were published between year 2000 and 2020 in domestic and foreign journals, using databases such as: PubMed, PubMed Central, Medline, EBSCO, Google Scholar and Embase with keywords: SALIVA, Dry Mouth, Xerostomia, SALIVAry Determination and SALIVA Estimation. Among the relevant authorities, the articles that were cited many times and were more up-to-date, were selected. Conclusion: It seems like using SALIVA in modern diagnostic tests, makes the diagnosis of many diseases easier, faster and more accurate. The standard methods of SALIVA collection in this article can be used as a guide for laboratory-clinical researchs by researchers.

Background and Aim: The microbial cause of dental caries has been proved among its multifactorial etiologies. The prevention and control of dental caries is very critical because of high prevalence and cost especially in high risk patient such as xerostomia. Based on the world attraction to traditional treatment and importance of drug extraction of natural material and plants, in this in vitro study effect of hydroalcoholic extract of Salvia officinalis and Menta longifolia which were very useful in traditional treatment of mouth and teeth disease has been determined against cariogenic bacteria.Materials and Methods: In this experimental study, hydroalcoholic extracts have been prepared from Salvia officinalis and Menta longifolia with maceration method. Their antibacterial activity against Streptococcus mutans, Lactobacillus rhamnosus and Actinomyces viscosus have been evaluated with broth macrodilution method. Results have been analyzed with Mann-Whitney test.Results: Minimum inhibitory concentration for Salvia officinalis and Menta longifolia for streptococcus mutans were respectively 6.25 and 12.5 mg/ml, for Lactobacillus rhamnosus 1.56 and 3.12 mg/ml and for Actinomyces viscosus 12.5 and 100 mg/ml.Conclusion: Both extracts had growth inhibitory effect on all three bacteria. SALIVA officinalis had greater effect on inhibition of growth of all three bacteria (p<0.05). Both extracts had bactericidal effect in the range of studied concentrations.