مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

78
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

34
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

Tracking the Transplanted Neurosphere in Retinal Pigment Epithelium Degeneration Model

Pages

  523-532

Abstract

 Introduction: Retinal Pigment Epithelium (RPE) layer deterioration is a leading cause of Age-Related macular degeneration (AMD), i. e., the most significant reason for irreversible blindness. The present study aimed to track the Neurosphere-Derived (NS) from Bone marrow stromal stem cells (BMSCs) grafted into the sub-retinal space (destruction of the RPE layer by Sodium iodate). Methods: RPE degeneration model was performed using the injection of 5% Sodium iodate performed in the retro-orbital sinus of Wistar rats. BMSCs were extracted from the examined rat femur and induced into NS, using EGF, bFGF, and B27. BrdU-NS labeled cells were transplanted into the sub-retinal space. For detecting BMSCs and NS markers, immunocytochemistry was performed. Moreover, immunohistochemical was conducted for tracking the transplanted cells in the RPE and sensory retina. Results: The immunocytochemistry of BMSCs cells displayed the expression of mesenchymal stem cells markers (CD90,99%±, 1), CD166 (98%±, 2), CD44 (99%±, 1). Additionally, the expression of neural lineage markers in NS, such as SOX2, OCT4, Nanog, Nestin, and Neurofilaments (68, 160, 200) revealed the differentiation from BMSCs. Tracking BrdU-NS labeled suggested these aggregations in most layers of the retina. Conclusion: Our study data indicated that BMSCs derived neurosphere had the potential to migrate in injured retinal and integrate into the neurosensory retina. These data can be useful in finding safe cells for replacement therapy in AMD.

Multimedia

  • No record.

    • Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    ABOUTALEB KADKHODAEIAN, HAMID, Salati, Amir, ANSARI, MOJTABA, & Taghdiri Nooshabadi, Vajihe. (2021). Tracking the Transplanted Neurosphere in Retinal Pigment Epithelium Degeneration Model. BASIC AND CLINICAL NEUROSCIENCE, 12(4), 523-532. SID. https://sid.ir/paper/990856/en

    Vancouver: Copy

    ABOUTALEB KADKHODAEIAN HAMID, Salati Amir, ANSARI MOJTABA, Taghdiri Nooshabadi Vajihe. Tracking the Transplanted Neurosphere in Retinal Pigment Epithelium Degeneration Model. BASIC AND CLINICAL NEUROSCIENCE[Internet]. 2021;12(4):523-532. Available from: https://sid.ir/paper/990856/en

    IEEE: Copy

    HAMID ABOUTALEB KADKHODAEIAN, Amir Salati, MOJTABA ANSARI, and Vajihe Taghdiri Nooshabadi, “Tracking the Transplanted Neurosphere in Retinal Pigment Epithelium Degeneration Model,” BASIC AND CLINICAL NEUROSCIENCE, vol. 12, no. 4, pp. 523–532, 2021, [Online]. Available: https://sid.ir/paper/990856/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button