IDENTIFICATION OF ILLEGAL DYES IN MUCOUS COSMETIC

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MALEKPOUR AKBAR; FATAHIAN MARYAM; HASANPOUR ZAHRA

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Seminar Paper

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Seminar: IRANIAN CHEMISTRY CONGRESS
Year:2013 | Issue:16

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Title

IDENTIFICATION OF ILLEGAL DYES IN MUCOUS COSMETIC

Author(s)

MALEKPOUR AKBAR | FATAHIAN MARYAM | HASANPOUR ZAHRA | Issue Writer Certificate

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Abstract

IN RECENT YEARS INCREASING ATTENTION HAS BEEN FOCUSED ON THE WIDESPREAD USE OF SYNTHETIC DYES, ESPECIALLY IN FOOD AND COSMETICS, BECAUSE OF THEIR POTENTIAL TOXICITY. IN REPUBLIC OF CHINA, THE FIRST GROUP OF 65DYES CAN BE USED IN COSMETICS, THE SECOND GROUP INCLUDING 25 DYES IS NOT ALLOWED TO BE USED IN MUCOUS COSMETICS. IN THIS STUDY WE EXPECTED TO SEPARATE AND IDENTIFY 25 DYES IN THE SECOND GROUP AS WELL AS AMARANTH (RED NO 2) WHICH WAS EXCLUDED IN USE FOR COSMETICS DUE TO SAFETY CONSIDERATION BY DEVELOPING A HPLC METHOD. ILLEGAL COAL TAR DYES ARE REPORTED TO POSSESS STRONG TOXICITY AND CARCINOGENICITY WHEN ADDED IN MUCOUS COSMETICS. LIPSTICK IS CLASSIFIED INTO THE SECOND CATEGORY BASED ON CHINES NATIONAL STANDARD (CNS) IN MUCOUS COSMETICS, IN ADDITION TO WAX LIPIDS, COLORING DYES ARE THE MOST IMPORTANT INGREDIENTS OF LIPSTICK, THE NEWLY DEVELOPED METHOD WAS USED TO ESTABLISH AN ANALYTICAL METHOD TO SIMPLIFY EXTRACTION AND IDENTIFICATION PROCEDURES FOR ANALYZING COLORING DYES IN COMMERCIAL LIPSTICK IN ORDER TO CHECK FOR ILLEGAL COLORING DYES.THE COLORANTS WERE EXTRACTED FROM THE COSMETIC PRODUCTS THROUGH TWO METHOD, ONE OF THEM WAS ADSORPTION OF CONTAMINANTS BY MACS) MAGNETIC ACTIVATED CARBONS) AND MAGNETIC SEPARATION AND ANOTHER ONE WAS LIQUID-LIQUID EXTRACTION THEN RE-EXTRACTED USING A COTTONDYEING PROCESS; THESE TWO METHODS USED FOR PRE-CONCENTRATION AND EXTRACTION OF COLORING DYES BOTH GREATLY IMPROVED ANALYTICAL EFFICIENCY AND PRECISION AND THE LIMIT OF DETECTION, FURTHERMORE TBA-BR WAS SELECTED AS ION-PAIR REAGENT IN THIS STUDY. RESULTS SHOWED THAT CAPACITY FACTORS OF DYES WERE IMPROVED WITH INCREASING TBA-BR CONCENTRATION WHICH THE OPTIMUM CONCENTRATION OF TBA-BR WAS FOUND TO BE 0.01 M. IN THIS STUDY WE USED HPLC WITH OPTIMAL CONDITIONS USING C18 COLUMN WITH THE MOBILE PHASE USING 0.07 M AMMONIUM ACETATE SOLUTION IN0.01 TETRABUTYLAMMONIUM BROMIDE /METHANOL/ACETONITRILE55: 35: 10 AND COLORANTS WHICH WERE ANALYZED BY HPLC FOLLOWED BY DAD DETECTOR. A WAVELENGTH AT 520 NM WAS FOUND TO BE MOST FAVORABLE FOR THE DETECTION OF RED COLOR, A MAJOR COLOR WIDELY APPLIED ON LIPSTICKS AND A WAVELENGTH AT 254 NM, WHICH BELONGED TO UV ABSORBANCE WAVELENGTH NORMALLY ABSORBED BY COAL TAR DYES, WAS USED TO DETECT THE COLOR OTHER THAN RED SUCH AS GREEN, ORANGE, YELLOW, BLACK, AND PURPLE IN COLOR. BY APPLYING THIS HPLC METHOD ALONG WITH THE ABOVE-MENTIONED PROCEDURE, WATER SOLUBLE COAL TAR DYE CONSTITUENTS CAN BE SATISFACTORILY SEPARATED WITH OTHER PREVIOUSLY MENTIONED PROFITS.

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APA: Copy

MALEKPOUR, AKBAR, FATAHIAN, MARYAM, & HASANPOUR, ZAHRA. (2013). IDENTIFICATION OF ILLEGAL DYES IN MUCOUS COSMETIC. IRANIAN CHEMISTRY CONGRESS. SID. https://sid.ir/paper/913944/en

Vancouver: Copy

MALEKPOUR AKBAR, FATAHIAN MARYAM, HASANPOUR ZAHRA. IDENTIFICATION OF ILLEGAL DYES IN MUCOUS COSMETIC. 2013. Available from: https://sid.ir/paper/913944/en

IEEE: Copy

AKBAR MALEKPOUR, MARYAM FATAHIAN, and ZAHRA HASANPOUR, “IDENTIFICATION OF ILLEGAL DYES IN MUCOUS COSMETIC,” presented at the IRANIAN CHEMISTRY CONGRESS. 2013, [Online]. Available: https://sid.ir/paper/913944/en

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