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Information Journal Paper

Title

Development of a Multi-Epitope Recombinant Protein for the Diagnosis of Human Visceral Leishmaniasis

Pages

  1-10

Abstract

 Background: Iran is one of the endemic areas of Mediterranean Visceral Leishmaniasis, a disease caused by Leishmania infantum. In this work, we examined whether Proteí na quimé rica 10 (PQ10) recombinant protein is suitable for immunological diagnosis of human visceral leishmaniasis. Methods: The study was carried out in Tarbiat Modares University during 2016-2018. The coding sequence of PQ10 recombinant protein was sub-cloned in pET28 expression vector and was commercially synthesized by GENERAY Biotechnology, China. Sequencing with proper primers was done, the expression, optimization of expression and protein purification were performed, and the purified recombinant protein was confirmed by western blot. The efficacy of PQ10 for serodiagnosis was evaluated with 50 positive and 50 negative serum samples, which confirmed by the direct agglutination test and collected from individuals living in the visceral leishmaniasis endemic areas of Iran. ELISA was performed with the PQ10 recombinant protein. Results: The 95% CI sensitivity of ELISA that was evaluated with sera from naturally infected individuals was 84%. The 95% CI specificity value of the ELISA determined with sera from healthy individuals (50 serum samples) and from individuals with other infectious diseases was 82%. The 95% CI positive predictive value (PPV) and negative predictive value (NPV) were exterminated 82. 35% and 83. 67%, respectively. Conclusion: We have used a recombinant synthetic protein to improve serodiagnosis of human visceral leishmaniasis. PQ10 could be useful for diagnosis of asymptomatic cases, as well as in the early phase of infections.

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    APA: Copy

    Jameie, Farnoosh, DALIMI, ABDOLHOSSEIN, PIRESTANI, MAJID, & MOHEBALI, MEHDI. (2021). Development of a Multi-Epitope Recombinant Protein for the Diagnosis of Human Visceral Leishmaniasis. IRANIAN JOURNAL OF PARASITOLOGY, 16(1), 1-10. SID. https://sid.ir/paper/679201/en

    Vancouver: Copy

    Jameie Farnoosh, DALIMI ABDOLHOSSEIN, PIRESTANI MAJID, MOHEBALI MEHDI. Development of a Multi-Epitope Recombinant Protein for the Diagnosis of Human Visceral Leishmaniasis. IRANIAN JOURNAL OF PARASITOLOGY[Internet]. 2021;16(1):1-10. Available from: https://sid.ir/paper/679201/en

    IEEE: Copy

    Farnoosh Jameie, ABDOLHOSSEIN DALIMI, MAJID PIRESTANI, and MEHDI MOHEBALI, “Development of a Multi-Epitope Recombinant Protein for the Diagnosis of Human Visceral Leishmaniasis,” IRANIAN JOURNAL OF PARASITOLOGY, vol. 16, no. 1, pp. 1–10, 2021, [Online]. Available: https://sid.ir/paper/679201/en

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