SUBSTRATE BINDING OF MALTOGENIC AMYLASE BASED ON MOLECULAR DOCKING SIMULATION

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SEPEHRI SEPIDEH; ARAB SEYED SHAHRIAR; HASAN SAJEDI REZA

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Paper Information

Journal: MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS Year:2014 | Volume:3 | Issue:SUPPL. (1) Page(s): 281-281

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Title

SUBSTRATE BINDING OF MALTOGENIC AMYLASE BASED ON MOLECULAR DOCKING SIMULATION

Author(s)

SEPEHRI SEPIDEH | ARAB SEYED SHAHRIAR | HASAN SAJEDI REZA

Keywords

MOLECULAR DOCKING

KINETIC PARAMETERS

MALTOGENIC AMYLASE

CYCLODEXTRINS

Abstract

Maltogenic amylases (MAase) are a group of cyclodextrin (CD) hydrolyzing enzymes which recently attract increasing amount of attention, due to its industrial applications in baking and confectionary industries and also its use in pharmaceuticals. We decided to investigate affinity of theGeobacillus sp. Gh6 MAase to various substrates by molecular docking simulation and compared with kinetic parameters. Molecular docking is a well-established computational technique which predicts the interaction energy between two molecules. Previous studies have shown a noticeable preference for a-, b - and g-CD compared to polymeric substrates (amylose and amylopectin) possibly due to steric interference. The Km for CD substrates increased in the order of a-CD>> b-CD>g-CD, and kcat/Km increased as a-CD> b -CD>g-CD, implying that increased substrate specificities are mainly attribute to kcat. In this study, binding of the enzyme to a-, b-, g-CD, amylose and amylopectin was studied using molecular docking and free energy of binding and dissociation constant was estimated. Interestingly, dissociation constant is highest for polymeric substrates compared to CDs and also a-CD>> b -CD>g-CD and order of the free energy of binding is amylopectin>amylose>>a-CD> b -CD>g-CD. All of the results showed that the affinity for substrates increased in the order of g-CD> b -CD>a-CD>>amylose>amylopectin.

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APA: Copy

SEPEHRI, SEPIDEH, ARAB, SEYED SHAHRIAR, & HASAN SAJEDI, REZA. (2014). SUBSTRATE BINDING OF MALTOGENIC AMYLASE BASED ON MOLECULAR DOCKING SIMULATION. MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS, 3(SUPPL. (1)), 281-281. SID. https://sid.ir/paper/335961/en

Vancouver: Copy

SEPEHRI SEPIDEH, ARAB SEYED SHAHRIAR, HASAN SAJEDI REZA. SUBSTRATE BINDING OF MALTOGENIC AMYLASE BASED ON MOLECULAR DOCKING SIMULATION. MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS[Internet]. 2014;3(SUPPL. (1)):281-281. Available from: https://sid.ir/paper/335961/en

IEEE: Copy

SEPIDEH SEPEHRI, SEYED SHAHRIAR ARAB, and REZA HASAN SAJEDI, “SUBSTRATE BINDING OF MALTOGENIC AMYLASE BASED ON MOLECULAR DOCKING SIMULATION,” MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS, vol. 3, no. SUPPL. (1), pp. 281–281, 2014, [Online]. Available: https://sid.ir/paper/335961/en

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