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Information Journal Paper

Title

Cupric Oxide Nanoparticles Induce Cellular Toxicity in Liver and Intestine Cell Lines

Pages

  213-220

Abstract

 Purpose: The wide application of cupric oxide nanoparticles (copper (II) oxide, CuO-NPs) in various fields has increased exposure to the kind of active nanomaterials, which can cause negative effects on human and environment health. Although CuO-NPs were reported to be harmful to human, there is still a lack information related to their toxic potentials. In the present study, the toxic potentials of CuO-NPs were evaluated in the liver (HepG2 hepatocarcinoma) and intestine (Caco-2 colorectal adenocarcinoma) cells. Methods: After the characterization of particles, cellular uptake and morphological changes were determined. The potential of cytotoxic, genotoxic, oxidative and apoptotic damage was investigated with several in vitro assays. Results: The average size of the nanoparticles was 34. 9 nm, about 2%-5% of the exposure dose was detected in the cells and mainly accumulated in different organelles, causing oxidative stress, cell damages, and death. The IC50 values were 10. 90 and 10. 04 µ g/mL by MTT assay, and 12. 19 and 12. 06 µ g/mL by neutral red uptake (NRU) assay, in HepG2 and Caco-2 cells respectively. Apoptosis assumes to the main cell death pathway; the apoptosis percentages were 52. 9% in HepG2 and 45. 5% in Caco-2 cells. Comet assay result shows that the highest exposure concentration (20 µ g/mL) causes tail intensities about 9. 6 and 41. 8%, in HepG2 and Caco-2 cells, respectively. Conclusion: CuO-NPs were found to cause significant cytotoxicity, genotoxicity, and oxidative and apoptotic effects in both cell lines. Indeed, CuO-NPs could be dangerous to human health even if their toxic mechanisms should be elucidated with further studies.

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    APA: Copy

    Abudayyak, Mahmoud, Guzel, Elif, & OZHAN, GUL. (2020). Cupric Oxide Nanoparticles Induce Cellular Toxicity in Liver and Intestine Cell Lines. ADVANCED PHARMACEUTICAL BULLETIN, 10(2), 213-220. SID. https://sid.ir/paper/331920/en

    Vancouver: Copy

    Abudayyak Mahmoud, Guzel Elif, OZHAN GUL. Cupric Oxide Nanoparticles Induce Cellular Toxicity in Liver and Intestine Cell Lines. ADVANCED PHARMACEUTICAL BULLETIN[Internet]. 2020;10(2):213-220. Available from: https://sid.ir/paper/331920/en

    IEEE: Copy

    Mahmoud Abudayyak, Elif Guzel, and GUL OZHAN, “Cupric Oxide Nanoparticles Induce Cellular Toxicity in Liver and Intestine Cell Lines,” ADVANCED PHARMACEUTICAL BULLETIN, vol. 10, no. 2, pp. 213–220, 2020, [Online]. Available: https://sid.ir/paper/331920/en

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