مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

424
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

142
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

ANALYSIS OF NATURALLY OCCURRING RESISTANT MUTATIONS TO HEPATITIS C VIRUS NS3 PROTEASE INHIBITORS: A PRELIMINARY STUDY IN SOUTH OF IRAN

Pages

  1-6

Abstract

 Background: Exploring the rate of naturally occurring NS3 protease mutants in HCV infected population is influential in the future therapeutic approaches.Objectives: This study explored naturally occurring resistant mutations to PROTEASE INHIBITORS in a pilot study.Patients and Methods: We analyzed NS3 gene sequences in 7 HCV infected patients, referred to the central liver center, south of Iran. The protease domain was amplified by PCR followed by product extraction. Amplified NS3 genes were cloned by TA/cloning system. For each patient, clonal-sequencing was performed to improve mutation detection sensitivity. Then, the obtained sequences were compared with the reference sequences and final phylogenic tree was constructed. Afterwards, the sequences were studied to investigate point mutations.Results: Phylogenetic analysis between reference and amplified sequences demonstrated high similarity of all sequences with genotype 1. Interestingly, crucial protease resistant mutations were detected in V36 and R155 positions in one patient’s sequence. Checking different clones of this patient confirmed V36L, as the dominant mutation while R155K was detected only in a few cases.Conclusions: As revealed, naturally occurring resistant mutations, especially R155K in protease sequence were identified in 1 out of the 7 patients, so the rate of such mutations is estimated to be high. It seems that checking HCV patients before protease inhibitor treatment are necessary in the region.

Multimedia

  • No record.

    • Cites

  • No record.

    • References

    Cite

    APA: Copy

    AFRASIABI, MOZHGAN, HOSSEINI, SEYED YOUNES, YAGHOBI, RAMIN, FATTAHI, MOHAMMAD REZA, ARDEBILI, MARYAM, & KHODADAD, MAHBOOBEH. (2015). ANALYSIS OF NATURALLY OCCURRING RESISTANT MUTATIONS TO HEPATITIS C VIRUS NS3 PROTEASE INHIBITORS: A PRELIMINARY STUDY IN SOUTH OF IRAN. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 8(10), 1-6. SID. https://sid.ir/paper/317466/en

    Vancouver: Copy

    AFRASIABI MOZHGAN, HOSSEINI SEYED YOUNES, YAGHOBI RAMIN, FATTAHI MOHAMMAD REZA, ARDEBILI MARYAM, KHODADAD MAHBOOBEH. ANALYSIS OF NATURALLY OCCURRING RESISTANT MUTATIONS TO HEPATITIS C VIRUS NS3 PROTEASE INHIBITORS: A PRELIMINARY STUDY IN SOUTH OF IRAN. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2015;8(10):1-6. Available from: https://sid.ir/paper/317466/en

    IEEE: Copy

    MOZHGAN AFRASIABI, SEYED YOUNES HOSSEINI, RAMIN YAGHOBI, MOHAMMAD REZA FATTAHI, MARYAM ARDEBILI, and MAHBOOBEH KHODADAD, “ANALYSIS OF NATURALLY OCCURRING RESISTANT MUTATIONS TO HEPATITIS C VIRUS NS3 PROTEASE INHIBITORS: A PRELIMINARY STUDY IN SOUTH OF IRAN,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 8, no. 10, pp. 1–6, 2015, [Online]. Available: https://sid.ir/paper/317466/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button