Investigation of the Relationship between Virus Neutralization Test and ELISA with Challenge Test in Evaluation of FMD Vaccine Potency

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Mahdieh Akbarzadegan Mahdieh Akbarzadegan; Homayoon Mahravani Homayoon Mahravani; Zeinab Piravar Zeinab Piravar

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Journal Paper

Paper Information

Journal: Iranian Journal of Virology Year:2023 | Volume:17 | Issue:1 Page(s): 57-62

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Title

Investigation of the Relationship between Virus Neutralization Test and ELISA with Challenge Test in Evaluation of FMD Vaccine Potency

Author(s)

Mahdieh Akbarzadegan Mahdieh Akbarzadegan | Homayoon Mahravani Homayoon Mahravani | Zeinab Piravar Zeinab Piravar | Issue Writer Certificate

Keywords

FMD

serum neutralization test

inactivated FMD vaccine

challenge test

determination of protective dose.

Abstract

Abstract Background and Aims: Foot-and-mouth disease (FMD), is a highly infectious and contagious disease in livestock. An effective and efficient vaccine is needed to Control FMD and reduces the associated damage. The goal of this study was to find out if the protective dose or challenge (PD50%) could be used instead of the antibody titer method to measure the effectiveness of the FMD vaccine. To achieve this, several calves were selected and divided into four groups. The vaccine was administered in three doses: a full dose, a 1/3 dose and a 1/9 dose. Twenty-one days after vaccination, all animals were challenged with a 10,000 LID50% virus in the tongue epithelium. For 7 days after, the animals were evaluated and monitored for the appearance of FMD symptoms. The PD50% (Protective Dose) for each vaccine against the virus was determined in the experiment. After obtaining the VN results that indicated the antibody titer and the PD50% level, a comparison was made between these two parameters. By examining 5 test cases, a formula was derived that accurately determined the PD50% with a high degree of precision using the VN50% result. This study determined the constants for A and O types using the VN50% test results. By incorporating these values into the derived formula, the PD50% level could be determined.

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