RNAi knockdown of heat shock proteins affects thermotolerance of Ephestia kuehniella (Lepidoptera: Pyralidae)

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Farahani Saeed; Bandani Ali Reza

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Journal Paper

Paper Information

Journal: JOURNAL OF CROP PROTECTION Year:2024 | Volume:13 | Issue:3 Page(s): 225-242

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Information Journal Paper

Title

RNAi knockdown of heat shock proteins affects thermotolerance of Ephestia kuehniella (Lepidoptera: Pyralidae)

Author(s)

Farahani Saeed | Bandani Ali Reza | Issue Writer Certificate

Keywords

HSP70

HSP90

RNA interference

Integrated pest management

Abstract

The Mediterranean flour moth, Ephestia kueheniella is one of the most severe pests in stores worldwide. Like many other stored product pests, chemical pesticides are often used to control this insect. As many of these chemicals negatively impact non-target species, safer alternative methods are needed. Temperature is one of the environmentally friendly control methods that can achieve the desired levels of control. Still, the insects' induction of cold and heat tolerance can decrease control efficacy. In the current study, we first investigated the susceptibility of all developmental stages of the Mediterranean flour moth to extreme heat and cold temperatures. Exposure to 44 °C for 80 minutes or -15 °C for 30 minutes caused significant mortalities in all developmental stages. Exposure to mild low or high temperatures induced cold or heat tolerance and reduced the mortality rate. Quantitative RT-PCR confirmed that heat or cold-inducible tolerance was accompanied by up-regulation of transcripts for two heat shock proteins, HSP70 and HSP90; transcript levels of HSP70 and HSP90 increased 3.8 and 4.3-fold after pre-exposure to 35°C for 30 min and 3.8 and 3.3-fold after pre-exposure to 10°C for 30 min compared to controls, respectively. Suppression of HSP70 and HSP90 transcripts using RNA interference (RNAi) significantly reduced heat and cold tolerance, indicating that these two proteins play a crucial role in the induction of heat and cold tolerance in the Mediterranean flour moth.

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