Background and Aim: Considering the lack of sufficient and even contradictory studies on the effect of temperature-dependent dimensional changes of alginate before pouring, this study aimed to investigate the effect of storage temperature of Hygedent and Chromogel alginate molds on dimensional stability. Materials and Methods: In this in-vitro experimental study, dimensional changes of two types of alginate were investigated indirectly by measuring a type III stone cast. Ninety molds of each alginate were obtained from a metal model and stored at 1±, 4, 1±, 25, and 1±, 40°, C. The casting was performed 12 minutes, 1 hour, and 4 hours after impression taking. The molds were incubated with an approximate humidity of 100%. The diameter, width, and height of the gypsum casts were measured by a digital caliper with an accuracy of 0. 01 mm. Dimensional changes of two alginates in three separate directions were statistically analyzed by three-way analysis of variance (ANOVA) and post-hoc test due to the significant difference. Result: There was no statistically significant difference in width changes at different temperatures, but regarding the diameter, there was a significant difference between 40°, C Chromogel and 25°, C Hygedent. Regarding the height, only 40°, C Hygedent showed a significant difference at 4 hours compared to 12 minutes (P=0. 000, 0. 26, and 0. 000, respectively). However, no statistically significant difference was observed in other temperatures and times (P>0. 05). In most groups, dimensional changes of all samples were not within the clinical standard range (1%). Conclusion: It seems that the dimensional stability of Hygedent and Chromogel alginates is affected by storage temperature, pouring time, and type of alginate.