Object: Determination of vitamin A and Beta carotene status in Holestein cow.Design: Cross sectional study. Animals: A total of 342 blood and 371 liver samples from Holestein cows in both sexes and 5 age groups as <2, 2-3, 3-4, 4-5, 5< years. Procedure: Livels of vit. A as well as R carotene were determined in serum and liver by a spectrophotometer at wave length of 325 - and 453nm as follows: Levels of vitamin A in serum and liver =[(0.000017×figure of beta carotene)-(absorption levels at 325nm)]/(0.00182) Levels of β carotene In serum and liver = (absorption levels 453 nm)/ 0.00258 Hexane was used as a blank. Statistical analysis: Results were analyzed by ANOVA. Results: In this survey the levels of vitamin A and Beta carotene of serum and liver were 67.2 ± 3.1, 85.5 ± 4.0 µg/dl and 189.3 ± 3.0, 21.1 ± 1 µg/g respectively. Differences of liver vitamin A levels in 4 and 5 age groups and also between these two age groups and others were significant (P<0.001). In the meanwhile levels of liver vitamin A and those of liver and serum β carotene of females were significantly higher than of males. Effects of season alone and both of season and sex, season and age, age and sex combinations were different. Apart from vitamin A and β carotene the relationship among all the other variants of this survey were significant. Conclusion: On the basis of the results of this survey effects of nutrition and to some extent female and vitamin A and βcarotene were highly considerable but high Levels of liver vitamin A in winter were not explainable.