From methanolic extract of aerial parts of Peucedanum RUTHENICUM M. Bieb (Apiaceae) collected from Mazandaran province of Iran, four flavonoids namely Isorhamnetin 3-0- rutinoside 1, rutin 2, quercetin 3, morin 4 and two phenolic acids namely caffeic acid 5 and p-coumaric acid 6 have been isolated by Paper Chromatography (PC) and crystallization. Their structures were elucidated by MS, 1H,  13C NMR spectra.

Antimicrobial activity of the polar and nonpolar extracts of the roots and aerial parts of Peucedanum RUTHENICUM M. Bieb., Johreniopsis seseloides (C. A. Mey.) Pimenov and Cervaria cervariifolia (C. A. Mey.) Pimenov were examined against gram-positive and gram-negative bacteria. The polar extracts of the roots and aerial parts displayed no activity against bacteria, while the nonoplar extracts of the roots which contains coumarins, presented activity against Staphylococcus aureus, S. epidermidis, Pseudomonas aeruginosa and Escherichia coli.

The essential oil of Peucedanum RUTHENICUM fruits, obtained by hydrodistillation, was analyzed by gas chromatography and gas chromatography-mass spectrometry. Among the 31 identified constituents accounting for 83.9% of the total oil, the major components were 1,8-cineole (11.15%), camphor (5.86%), Z-carveol (6.88%), lcarvone (5.61%), 8,9-dehydroisolongifolene (11.35%), caryophyllene oxide (13.65%), and caryophylla-4(12),8(13)-dien-5-β-ol (5.19%). Antimicrobial activity of the essential oil was investigated against various gram-positive and gram-negative bacteria. The essential oil of P. RUTHENICUM showed activity against gram-positive bacteria but had no effect on the tested gram negative bacteria.

The essential oils of Peucedanum RUTHENICUM M. Bieb. (Umbelliferae) leaves, flowers and fruits were prepared by hydrodistillation and analyzed by GC and GC-MS and the composition of the three essential oils were compared. Thirty-three, thirty-seven and thirty-one compounds were identified in leaves, flowers and fruits essential oils representing 89.13%, 88.66% and 76.45% of total oils respectively. The major components were thymol (18.29%), b -bisabulene (13.29%) for leaves oil, b-myrcene (10.68 %) and germacrene-B (10.06%) for flowers oil and caryophyllene oxide (13.65%), 8, 9-dehydroisolongifolene (11.33%) and 1, 8-cineol (11.15%) for fruits oil. The amounts of monoterpens and sesquiterpenes were found nearly to be equal in oils of the three parts of plant.