Radiotherapy has a profound impact on ovarian function, leading to depletion of the primordial follicle reserve, premature amenorrhea, and loss of fertility during or shortly after completion of irradiation. The radioprotectors are compounds that can reduce the effects of ionizing radiation (IR). Stabilized selenium nanoparticles and extracts, including ROSEMARY, demonstrated high antioxidant activity. The aim of this study is the evaluation of ROSEMARY extract and selenium nanoparticles ability to scavenge free radicals. After ROSEMARY extract was prepared, nanoparticles were made in their presence, and then CHO cells were cultured in vitro. They were then divided into different groups and treated with different concentrations of nanoparticles and ROSEMARY, and irradiated with ionizing radiation at doses of 0.5, 1, and 2. Finally, the survival rate was measured by MTT assay. The results of the MTT test showed that the effect of radiation protection on nanoparticles is close to ROSEMARY. Furthermore, the protective effect of the two is not significantly different from each other (P>0.05). Contrary to expectations, nanoparticles did not show a synergistic effect compared to ROSEMARY.

Introduction: The use of nanotechnology in drug delivery to the cells can somewhat address some of the problems involved in the delivery of herbal medicines, including non-targeting and high oxidizing features. Therefore, this study aimed to investigate the niosomal nano-carriers containing Rosmarinus officinalisessential oil to improve itsphysicochemical characteristics and increase its antitumor properties. Materials & Methods: The vesicles of niosome were preparedby thin-film method using span 60, cholesterol and polyethylene glycol and the Rosmarinus officinalisessential oil were loaded into the niosomes. Thereafter, theirphysicochemical characteristics were evaluated using Zeta Sizer, FTIR, SEM, and the essential oil release amount was calculated at 37° C and 42° C. Finally, MTT assay was performed to assess the toxicity of the nano-carrier containingessential oil against the KG-1 cell line of Acute Myeloid Leukemia (AML). Findings: The results of the present study indicated thatprepared nano-carriers containing essential oil have encapsulation efficiency, size and zeta potential include 86. 75, 73. 6 nm and-45. 46 mV, respectively. SEM images have indicated the spherical morphology of nanoniosomes. Furthermore, the results showed the appropriate position of the essential oil in the nano-carriers and controlled release at 37oC and 42oC. Additionally, MTT assay results demonstrate that the encapsulated essential oil has a higher toxicity against KG-1 cell line than the free essential oil. Discussion & Conclusions: The results of this study, while confirming the method used for niosomesynthesizing, the use of niosome as an effective carrier for essential oil encapsulation, andits slow-releasing feature, suggests that this nanosystem can increase its stability without altering the chemical nature of the essential oil. Accordingly, it can be suitable for the delivery of ROSEMARY essential oils to KG-1 cells the and the exertion of its effects on the cells.

Background:ROSEMARY is an evergreen and aromatic plant of the mint family, which has many antioxidant and medicinal properties. Nowadays, to increase the quantity of extraction and improve the quality of essence, the plant is dried and then the extraction process is performed. Thus, new and combined methods for drying of plants containing essence have been studied. Different drying methods such as hot air, microwave, microwave- vacuum, sun and freeze drying have been studied by many researcher to dry ROSEMARY and the quality of the extracted essence and the kinetics of mass transfer have been investigated. Drying by means of infrared is considered for many agricultural products but this method has not been applied to ROSEMARY until now. Therefore, the purpose of this study was to investigate the kinetics of drying and the effect of infrared drying on the volume, color and quality of essence of ROSEMARY leaves. Our specific target was modeling of the infrared drying process and compare it with other drying methods. Materials and Methods: In this study, the geometric properties of ROSEMARY leaves were measured by Mitutoyo Micrometer. Then, by using electromagnetic radiation in the range of infrared spectrum (100, 200 and 300watts) freshly prepared leaves of ROSEMARY were dried. After essence extraction by clevenger method, the volume of extraction and components were determined by GC/MS and according to the power of infrared wavelength were evaluated by completely randomized design. The quality of dried ROSEMARY and essences were also studied by ImageJ software, and color parameters a*, b*, L*, chroma (C*), browning index, total color difference and Hue angle, were calculated by completely randomized design. In addition, drying curves, effective diffusivities and activation energy determined by using moisture measurement data.Results:According to the results of analysis of variance and Duncan's Multiple Range test at 5% level, it was observed that with increasing the power of the infrared lamp in the drying process, color parameters like L, b, Chroma and BI in dried ROSEMARY decreased and ΔE, A and Hue angel increased. Analysis of variance and comparison of Duncan's mean at 5% level showed that increasing the infrared power has a significant effect on reducing the volume of essence. In the case of essence color, it was perceived that with increasing the power of the infrared lamp, the color parameters like A, L and Hue angel decreased and b, BI and Chroma increased. Furthermore, increasing the infrared power in the drying process of ROSEMARY has a significant effect on reducing the volatile compounds of ROSEMARY essence. Effective diffusivities varied from 33.3×9.10-9 to 1.0907×10-8. The activation energy was also determined as 30.243 kW/kg. To predict the ROSEMARY drying trend by using eight models, regression modeling was performed through MATLAB software (version 2016). Results showed that, Midilli model for the power of 200 and 300 watts and Verma model for the power of 100 watts, due to high correlation coefficient index and low standard error, were two suitable models for evaluation of Drying kinetic and prediction of drying process.Conclusion:Increasing infrared power during drying process of ROSEMARY leaves decreases the efficiency of essence extraction. Moreover, increasing the infrared power has a significant effect on reducing the active ingredients like cineol and Beta-Pinene in essence. The color of dried ROSEMARY and extracted essence are affected by infrared radiation power and varies with increasing the power. Rising the infrared radiation power results in an increase in the effective diffusivity coefficients. Also, it can be concluded that, midilli and verma are two best models for prediction of drying trend of ROSEMARY.