The aim of the present study was the histological evaluation of Enamel MATRIX Derivative (EMD) effectiveness for regeneration of periodontal defects. EMD activates cementum synthesis, PDL and bone during the maturation stage of follicole. In this research, EMD was used in surgical defects of premolar teeth in four adult sheep. Muccoperiosteal flap was reflected in buccal site of teeth. The buccal bone plate was removed from mesial to distal in 4 mm depth. After eliminating the cementum by bur and its etching, EMD was applied on exposed dentine and flap was sutured. In opposite sites of those teeth (control sites) the same process was performed without etching. After 100 days, sheep were sacrificed and histological study through light microscopic was performed on black sections of operation sites. The results showed that in test sites, regeneration of cementum and bone was 62/5% and 42/5-50% respectively. But in control sites regeneration of cementum and bone was 37.5% and 32/5-42/5% respectively. Also the migration of junctional epithelium in control sites was 8-10% more than test sites. The important point is that in test sites, cementum was completely attached to undermining dentine. But, in control sites, the gap between cementum and dentine was visible. As a result, this study suggests that EMD promotes periodontal regeneration, and EMD application is a successful achievement in regenerative periodontal therapy.

Different ossificant materials have been used used for induction of bone repair in many studies, and bone MATRIX gelatin which contains bone morphogenic proteins one of the best ones.In present study we evaluated the role of this mat erial in acceleration of bone repair in rabbit tibia.A hole of 305mm diameter was made on right tibia of 10 and 12 rabbits as study and control group respectively, In the experiment group, in addittion to Bone Wax, we applied bone MATRIX glatin in the hole. Radiographic images were taken in days 0, 20, 40 and 53 after operation In 6 rabbits of each group, photographic pictures were also taken after exposure of entire bone. In 6 controls less degree of restoration were seen on day 53. In 4 experimental animals restoration were completed by this time and in 2 speciments repair processing were better then controls. This results shows that bone MATRIX gelatin can be used as an acclerator of bone repair.

Background and aim: Palatal groove as a growth-developmental anomaly can lead to periodontal problems, therefore diagnosis and treatment of its complications is of high importance.The aim of this study was to evaluate clinically the efficacy of enamel MATRIX derivative (EMD) in the treatment of periodontal defects due to palatal groove.Materials and Methods: Ten patients with palatal groove on maxillary lateral incisor with pocket depth ≥4mm were chosen from those referred to department of periodontics, Faculty of Dentistry Tehran University of Medical Sciences, and private office (2002-2003). During the surgical procedure, a full thickness flap was elevated and respective root thoroughly planed. After groove saucerization, EMD was used to cover the defect. The flap was then coronally positioned and secured with 3-0 silk sutures.Clinical measurements were taken at baseline, 1 and 3 months post-surgery. These parameters included pocket probing depth (PPD) and clinical attachment level (CAL). Statistical analysis was performed by the test of difference between means.Results: mean PPD and CAL before surgery were 5.7mm and 6.2mm, respectively that changed to 1.5mm and 2.05mm at 3 months post-surgery-statistically significant gain of attachment (71.05%) was observed 3 months after surgery (p-value <0.000).Conclusion: The results of this study suggest that EMD can be used successfully for periodontal attachment gain in teeth with palatal groove.

Background and Aim: In spite of the achievements in the field of dental adhesives, we are facing challenges with dentine bonding resistance, strength and stability. According to recent studies the role of MMP inhibitors in association with bonding,s persistence and leakage reduction and restoration,s persistence is important. The aim of this study was to investigate the effect of doxycycline as a MMP inhibitor on the degree of conversion (DC) of an experimental dental adhesive.Materials and Methods: In this experimental study, a new dental adhesive blend was prepared by mixing doxycycline monohydrate (in concentrations of 0.0, 0.25, 0.5, and 1 wt.%) with monomers. The monomers were composed of 12% Bis-GMA and 10% TMPTMA, 28% HEMA, and 50% Ethanol by weight for all groups. Comphorquinone and amines were chosen as photo initiator system. Degree of conversion of all adhesives was measured using FTIR spectroscopy. The results were analyzed using one-way ANOVA and Tukey post hoc tests.Results: The results showed that addition of 0.25, 0.5, and 1 weight percent doxycycline did not significantly reduce the DC of the adhesives compared to 0.0% control group (p>0.05%).Conclusion: According to the results of this study, adding doxycycline to the adhesives did not adversely affect the DC.