The Laelapidae is a large family with worldwide distribution. Many Laelapids are ectoparasitic on small mammals or associated with arthropods, although some species are free living and predators especially in soil habitat; also they are common in stored products. During three years (2007-2009) of faunestic survey, samples were collected from different habitats such as soil, plant litters and stored products in Guilan Province. A total of 17 species from 5 genera and 2 subfamilies were collected and identified. Among the identified species, 4 species are new records for Iran and 10 species for Guilan province mite fauna. Moreover, the taxonomic characteristics of four new species for Iran mite fauna are mentioned. A key is also provided for Guilan Laelapid mite species. The list of identified species is as follows, new species for Iran mite fauna are marked by an asterisk.Subfamily Laelapinae Berlese: A-GenusAndrolaelaps Berlese [1-Androlaelaps casalis (Berlese) B-Genus HypoaspisCanestrini [2-Hypoaspis vacua (Michael) 3-H. lutegiensis* Shcherbak 4-H. karawaiewi (Berlese) 5-H. sclerotarsaCosta 6-H. lubrica Voigts and Oudemans 7-H. nolli Karg 8-H. queenslandica (Womersley) 9-H. angustiscutata* Willmann 10-H. aculeifer (Canestrini) 11-H. minor Costa 12-H. kargi Costa 13-H. astronomica* (Koch) 14-Hypoaspis (Laelaspis) austriaca* Sellnick C-Genus Stratiolaelaps Berlese [15-Stratiolaelaps miles(Berlese)].Subfamily Haemogamasinae Oudemans: D-Genus Eulaelaps Berlese [16- Eulaelaps stabularis (Koch)] E-Genus Haemogamasus Berlese [17-Haemogamasus pontiger (Berlese).].