This study was carried out to determine the olfactory preference of the Persian sturgeon, Acipenser persicus, of 7-9g to 20 free amino acids, using one positive control and one negative control, totaling 22 treatments. The trials were done in 10 replicates.In the qualitative evaluation of juvenile responses, alanine and to a lesser extent glycine stimulated the strong and obvious feeding (searching) reaction that was also conformed by quantitative evaluation.The amino acids of leucine, histidine, cysteine, also isoleucine and proline promoted a low positive response. The quantitative evaluation verified the result, except for proline. Aspartic acid, tyrosine, glutamic acid and threonine stimulated negative responses as repelling agents. The result of quantitative assay showed the highest negative effect in the case of glutamic acid (p<0.01). Alanine and glycine could be, therefore, considered as appellants and glutamic acid as a repellent for the Persian sturgeon.