PRECONCENTRATION AND DETERMINATION OF B-CAROTENE NANOPARTICLES IN FRUIT AND JUICE SAMPLES BASED ON MICELLAR MEDIATED SYSTEM

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POURREZA NAHID; NAGHDI TINA

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Seminar Paper

Paper Information

Seminar: IRANIAN SEMINAR OF ANALYTICAL CHEMISTRY
Year:2014 | Issue:20

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Title

PRECONCENTRATION AND DETERMINATION OF B-CAROTENE NANOPARTICLES IN FRUIT AND JUICE SAMPLES BASED ON MICELLAR MEDIATED SYSTEM

Author(s)

POURREZA NAHID | NAGHDI TINA | Issue Writer Certificate

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Abstract

B-CAROTENE IS A PRECURSOR OF VITAMIN A AND PLAYS AN IMPORTANT ROLE IN THE METABOLISM AND HUMAN HEALTH. HOWEVER, B -CAROTENE CAN BE DESTROYED IN FOOD PROCESSING METHOD AT HIGH TEMPERATURE AND IT DOES NOT DISSOLVE IN WATER WELL. HOWEVER, THE NANOMATERIAL FORM OF B -CAROTENE COULD PROTECT IT FROM HEAT AND MAKE B -CAROTENE BE EASILY DISSOLVED IN WATER. OVER THE LAST FEW YEARS B -CAROTENE NANO DISPERSIONS HAVE FOUND A WIDE RANGE OF APPLICATIONS IN FOOD, PHARMACEUTICAL AND CHEMICAL INDUSTRIES AS WELL AS AGRICULTURE AND ENVIRONMENTAL ENGINEERING [1, 2]. CLOUD POINT EXTRACTION (CPE) HAS BECOME ONE OF THE MOST OUTSTANDING EXTRACTION TECHNIQUES FOR DETERMINATION OF ANALYTES. CPE IS BASED ON THE SEPARATION OF A HOMOGENEOUS MICELLAR AQUEOUS SOLUTION INTO TWO ISOTROPIC LIQUID PHASES [3]. THIS PAPER PRESENTS A SIMPLE, GREEN AND LOW COST ANALYTICAL METHOD FOR PRECONCENTRATION AND DETERMINATION OF B -CAROTENE NANOPARTICLES CONTENT IN SOME FRUIT AND JUICE SAMPLES. THE PROPOSED METHOD FOR PRECONCENTRATION AND DETERMINATION OF B-CAROTENE NANOPARTICLES IS BASED ON THE ENRICHMENT OF B -CAROTENE NANOPARTICLES IN NONIONIC SURFACTANT PHASE WITH CLOUD POINT EXTRACTION FOLLOWED BY UV-VIS SPECTROPHOTOMETRIC DETERMINATION AT L=468 NM. THE INFLUENCE OF CHEMICAL VARIABLES SUCH AS PH OF THE SAMPLE SOLUTION, TYPE AND VOLUME OF THE BUFFER, ELECTROLYTE TYPE AND CONCENTRATION, SURFACTANT CONCENTRATION, TEMPERATURE AND INCUBATION TIME WAS STUDIED.UNDER THE OPTIMUM CONDITIONS THE METHOD YIELDED A LINEAR CALIBRATION CURVE IN THE RANGE OF 0.05-5 NG ML-1 FOR B -CAROTENE NANOPARTICLES (R=0.9992). FOR ROBUSTNESS, SAMPLES CONSIDERED FOR ANALYSIS WITH THIS ANALYTICAL METHOD WERE: CARROT, POTATO, WATERMELON, TOMATO FRUITS AND CHERRY AND ORANGE JUICES. THE RECOVERIES OF THE ANALYTE IN SPIKED SAMPLES WERE IN THE RANGE OF 96-105%, WITH RELATIVE STANDARD DEVIATION (RSD) OF 3.2% AND 5.6%.

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APA: Copy

POURREZA, NAHID, & NAGHDI, TINA. (2014). PRECONCENTRATION AND DETERMINATION OF B-CAROTENE NANOPARTICLES IN FRUIT AND JUICE SAMPLES BASED ON MICELLAR MEDIATED SYSTEM. IRANIAN SEMINAR OF ANALYTICAL CHEMISTRY. SID. https://sid.ir/paper/911196/en

Vancouver: Copy

POURREZA NAHID, NAGHDI TINA. PRECONCENTRATION AND DETERMINATION OF B-CAROTENE NANOPARTICLES IN FRUIT AND JUICE SAMPLES BASED ON MICELLAR MEDIATED SYSTEM. 2014. Available from: https://sid.ir/paper/911196/en

IEEE: Copy

NAHID POURREZA, and TINA NAGHDI, “PRECONCENTRATION AND DETERMINATION OF B-CAROTENE NANOPARTICLES IN FRUIT AND JUICE SAMPLES BASED ON MICELLAR MEDIATED SYSTEM,” presented at the IRANIAN SEMINAR OF ANALYTICAL CHEMISTRY. 2014, [Online]. Available: https://sid.ir/paper/911196/en

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