APPLICATION OF NANOPARTICLES IN PATHOGEN DETECTION

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SOLTANI N.; KARBALAEI HEIDARI H.R.

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Seminar Paper

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Seminar: IRANIAN SEMINAR OF ANALYTICAL CHEMISTRY
Year:2014 | Issue:20

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Title

APPLICATION OF NANOPARTICLES IN PATHOGEN DETECTION

Author(s)

SOLTANI N. | KARBALAEI HEIDARI H.R. | Issue Writer Certificate

Keywords

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Abstract

NANOPARTICLE-BASED DETECTION STRATEGY CAN PROVIDE AN EASY, CONVENIENT, AND RAPID SENSING METHOD FOR A WIDE RANGE OF BACTERIAL PATHOGENS. THE RAPID AND SENSITIVE DETECTION OF BACTERIA IS EXTREMELY IMPORTANT IN THE FIELDS OF BIOTECHNOLOGY, MEDICAL DIAGNOSIS, AND FOOD SAFETY. IN RECENT YEARS, BIOSENSORS HAVE BEEN EXPLORED FOR THEIR APPLICATION IN THE DETECTION OF PATHOGENIC BACTERIA [1].GOLD NANOPARTICLES (AUNPS) ARE AMONG ONE OF THE MOST EXTENSIVELY STUDIED NANOMATERIALS.GOLD NANOPARTICLES HAS BEEN WIDELY USED TO IMPROVE THE DETECTION SENSITIVITY IN VARIOUS ANALYTICAL APPLICATION. GOLD NANOPARTICLES EXHIBIT MANY UNIQUE AND INTERESTING PHYSICAL AND OPTICAL PROPERTIES SUCH AS SURFACE PLASMON RESONANCE (SPR). THE SURFACE PLASMON RESONANCE OF GOLD NANOPARTICLES IS AN OPTICAL PROPERTY THAT HOLDS PROMISING POTENTIALS IN BIOSENSING, MOLECULAR IMAGING, AND PHOTOTHERMAL TREATMENT OF DISEASES [2]. THE COMBINATION OF SPECTROSCOPY DETECTION METHODS AND AUNPS PROVIDES A PROMISING PLATFORM FOR THE DEVELOPMENT OF HIGHLY SENSITIVE AND CONVENIENT BIOSENSORS. WE HEREIN PRESENT IN OUR STUDY OF USING UV–VIS ABSORPTION SPECTROSCOPY AND TRANSMISSION ELECTRON MICROSCOPY (TEM) TO DETERMINE THE INTERACTION BETWEEN AUNPS WITH BACTERIA. AUNPS WERE APPLIED TO DETECTION OF SOME PATHOGENIC AND NON-PATHOGENIC MICROORGANISMS. VARIOUS BACTERIAL GENUS SUCH AS STAPHYLOCOCCUS AUREUS ATCC 25123, ESCHERICHIA COLI ATCC 25922, PSEUDOMONAS AERUGINOSA ATCC 27853, SERRATIA MARCESCENS, SALMONELLA TYPHI, KLEBSIELLA PNEUMONIA, SHIGELLA, BACILLUS LICHENIFORMIS, PROTEUS VULGARIS, AND BACILLUS SUBTILIS WERE CULTURED AND THEIR INTERACTIONS WITH THE SYNTHESIZED AUNPS WERE EVALUATED.WHEN BACTERIAL SAMPLES WERE MIXED WITH DIFFERENT SYNTHESIZED AUNPS, THE COLOR OF AUNPS WERE CHANGED FROM RED TO VIOLET THAT CAN BE SEEN BY THE NAKED EYE. THE AGGREGATION PHENOMENA WERE INVESTIGATED BY UV–VIS SPECTROSCOPY AND. TEM. THIS METHOD HAS PROVED TO BE APPLICABLE FOR COLORIMETRIC SENSING OF SOME PATHOGEN BACTERIA.

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APA: Copy

SOLTANI, N., & KARBALAEI HEIDARI, H.R.. (2014). APPLICATION OF NANOPARTICLES IN PATHOGEN DETECTION. IRANIAN SEMINAR OF ANALYTICAL CHEMISTRY. SID. https://sid.ir/paper/910772/en

Vancouver: Copy

SOLTANI N., KARBALAEI HEIDARI H.R.. APPLICATION OF NANOPARTICLES IN PATHOGEN DETECTION. 2014. Available from: https://sid.ir/paper/910772/en

IEEE: Copy

N. SOLTANI, and H.R. KARBALAEI HEIDARI, “APPLICATION OF NANOPARTICLES IN PATHOGEN DETECTION,” presented at the IRANIAN SEMINAR OF ANALYTICAL CHEMISTRY. 2014, [Online]. Available: https://sid.ir/paper/910772/en

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