IDENTIFICATION OF SELF-INCOMPATIBILITY ALLELES IN SOME IRANIAN AND FOREIGN ALMOND (PRUNUS DULCIS M.) CULTIVARS USING PCR

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VALIZADEH B.; ERSHADI AHMAD; GHOLAMI MANSOUR

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Journal: IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY Year:2008 | Volume:8 | Issue:4 Page(s): 249-258

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Title

IDENTIFICATION OF SELF-INCOMPATIBILITY ALLELES IN SOME IRANIAN AND FOREIGN ALMOND (PRUNUS DULCIS M.) CULTIVARS USING PCR

Author(s)

VALIZADEH B. | ERSHADI AHMAD | GHOLAMI MANSOUR | Issue Writer Certificate

Keywords

ALMONDQ3

SELF-INCOMPATIBILITY ALLELEQ2

PCRQ3

Abstract

Gametophytic self-incompatibility of almond (Prunus dulcis M) controlled by a single locus with several allelic variants. In this study, the S-alleles of 16 Iranian and 6 foreign almond cultivars were investigated by single and multiplex PCR technique with different set of oligonucleotide primers. Application of AS1II and AmyC5R primers with single PCR resulted in amplification of 9 known self-incompatibility alleles S1, S2, S3, S5/10, S7, S9, S11, S12, and S13. Four bands with different sizes from PCR products of known S-alleles were detected in Iranian almond cultivars (Mamaei, Raby, Sahand, Shirbadam, Hajmirzaei and Khorshidi) which may be related to new S-alleles. Multiplex PCR using the AS1II, AmyC5R and CEBASF primers verified the results of single PCR. Furthermore, it was also able to identify Sf-allele in self-compatible cultivars such as Tuono, Genco and Falsa Barese. From 32 S-alleles in Iranian almond cultivars only 28 were identified by using these primers. Two S-alleles for 12 cultivars and one S-allele for four cultivars were identified. S2, S7, S13, S3 and S10 alleles were more frequent than others in Iranian almond cultivars. This technique provides a precise and rapid method for identifying S-alleles from the genomic DNAs of almond cultivars.

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بررسی آلل های خودناسازگار در 32 رقم سیب ایرانی (Malus x domestica B) با استفاده از ایزوآنزیم های ریبونوکلئازهای خامه گل

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APA: Copy

VALIZADEH, B., ERSHADI, AHMAD, & GHOLAMI, MANSOUR. (2008). IDENTIFICATION OF SELF-INCOMPATIBILITY ALLELES IN SOME IRANIAN AND FOREIGN ALMOND (PRUNUS DULCIS M.) CULTIVARS USING PCR. IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, 8(4), 249-258. SID. https://sid.ir/paper/80807/en

Vancouver: Copy

VALIZADEH B., ERSHADI AHMAD, GHOLAMI MANSOUR. IDENTIFICATION OF SELF-INCOMPATIBILITY ALLELES IN SOME IRANIAN AND FOREIGN ALMOND (PRUNUS DULCIS M.) CULTIVARS USING PCR. IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY[Internet]. 2008;8(4):249-258. Available from: https://sid.ir/paper/80807/en

IEEE: Copy

B. VALIZADEH, AHMAD ERSHADI, and MANSOUR GHOLAMI, “IDENTIFICATION OF SELF-INCOMPATIBILITY ALLELES IN SOME IRANIAN AND FOREIGN ALMOND (PRUNUS DULCIS M.) CULTIVARS USING PCR,” IRANIAN JOURNAL OF HORTICULTURAL SCIENCE AND TECHNOLOGY, vol. 8, no. 4, pp. 249–258, 2008, [Online]. Available: https://sid.ir/paper/80807/en

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