APPLICATION OF REPETITIVE EXTRAGENIC PALINDROMIC ELEMENTS BASED ON PCR IN DETECTION OF GENETIC RELATIONSHIP OF LACTIC ACID BACTERIA SPECIES ISOLATED FROM TRADITIONAL FERMENTED FOOD PRODUCTS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

TAFVIZI F.; TAJABADI EBRAHIMI M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Agricultural Science and Technology Year:2015 | Volume:17 | Issue:1 Page(s): 87-98

Download Full-Text

Persian Verion

View:

708

Download:

226

Cites:

Information Journal Paper

Title

APPLICATION OF REPETITIVE EXTRAGENIC PALINDROMIC ELEMENTS BASED ON PCR IN DETECTION OF GENETIC RELATIONSHIP OF LACTIC ACID BACTERIA SPECIES ISOLATED FROM TRADITIONAL FERMENTED FOOD PRODUCTS

Author(s)

TAFVIZI F. | TAJABADI EBRAHIMI M. | Issue Writer Certificate

Keywords

16S RDNA SEQUENCINGQ3

REP-PCR FINGERPRINTINGQ3

Abstract

Tarkhineh is one of the traditional foods in Iran and a rich source of probiotic bacteria. The objective of this study was to identify the probiotic bacteria isolated from Tarkhineh using 16S rRNA gene sequencing and molecular typing with repetitive extragenic palindromic–PCR (REP-PCR). In total, 20 different bacteria were isolated from traditional dairy products and Tarkhineh. Molecular identification of the isolates was carried out by 16S rRNA gene sequencing, and DNA sequences of isolates deposited in GenBank. The REP-PCR reaction by REP1R-I, REP2-I and REP1R-I+REP2-I markers was performed for fingerprinting and characterization of the isolates. Unweighted pair group method with arithmetic mean (UPGMA) clustering methods were performed based on Dice similarity. The REP1R-I primer grouped isolates into three, and REP2-I and REP1R-I+REP2-I grouped all isolates into four main clusters in dendrograms. In all analyses, isolates of Lactobacillus casei, Lactobacillus brevis, Lactobacillus plantarum, and Entrococcus facium formed separate clusters. The results of sequencing corresponded to clustering in the dendrogram. According to the results, REPPCR is an accurate technique for determining the genetic diversity of lactic acid bacteria species.

Multimedia

No record.

Cites

No record.

References

No record.

Cite

APA: Copy

TAFVIZI, F., & TAJABADI EBRAHIMI, M.. (2015). APPLICATION OF REPETITIVE EXTRAGENIC PALINDROMIC ELEMENTS BASED ON PCR IN DETECTION OF GENETIC RELATIONSHIP OF LACTIC ACID BACTERIA SPECIES ISOLATED FROM TRADITIONAL FERMENTED FOOD PRODUCTS. JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY (JAST), 17(1), 87-98. SID. https://sid.ir/paper/63044/en

Vancouver: Copy

TAFVIZI F., TAJABADI EBRAHIMI M.. APPLICATION OF REPETITIVE EXTRAGENIC PALINDROMIC ELEMENTS BASED ON PCR IN DETECTION OF GENETIC RELATIONSHIP OF LACTIC ACID BACTERIA SPECIES ISOLATED FROM TRADITIONAL FERMENTED FOOD PRODUCTS. JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY (JAST)[Internet]. 2015;17(1):87-98. Available from: https://sid.ir/paper/63044/en

IEEE: Copy

F. TAFVIZI, and M. TAJABADI EBRAHIMI, “APPLICATION OF REPETITIVE EXTRAGENIC PALINDROMIC ELEMENTS BASED ON PCR IN DETECTION OF GENETIC RELATIONSHIP OF LACTIC ACID BACTERIA SPECIES ISOLATED FROM TRADITIONAL FERMENTED FOOD PRODUCTS,” JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY (JAST), vol. 17, no. 1, pp. 87–98, 2015, [Online]. Available: https://sid.ir/paper/63044/en

Related Journal Papers