GUM TRAGACANTH GELS AS A NEW SUPPORTING MATRIX FOR IMMOBILIZATION OF WHOLE-CELL

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

OTADY M.; VAZIRI A.; SEYF KORDI A.A.; KHEYR ALOMOUM A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Chemistry and Chemical Engineering Year:2005 | Volume:24 | Issue:4 Page(s): 1-7

Download Full-Text

View:

432

Download:

250

Cites:

Information Journal Paper

Title

GUM TRAGACANTH GELS AS A NEW SUPPORTING MATRIX FOR IMMOBILIZATION OF WHOLE-CELL

Author(s)

OTADY M. | VAZIRI A. | SEYF KORDI A.A. | KHEYR ALOMOUM A. | Issue Writer Certificate

Keywords

GUM TRAGACANTH

IMMOBILIZED WHOLE-CELL

PENICILLIN G ACYLASE

IONOMER

CROSSLINK

Abstract

We introduce a new smooth, non-toxic, biocompatible method for cross-linking of GUM TRAGACANTH (GT), a polysaccharide of natural origin, in order to serve as a new supporting matrix for immobilization systems. The modified gum is used as a matrix for the catalysis of the conversion of benzyl penicillin to 6-aminopenicillanic acid (6-APA) by means of Escherichia coli ATCC11105 with PENICILLIN G ACYLASE (PGA) activity. The results show that GT beads can not only serve as a proper matrix for immobilization, but show enhanced hydrolysis rate and stability compared to other immobilization systems used for this reaction. This signifies the potential of GT as a biocompatible matrix for immobilization and its positive prospects for use in more demanding immobilization applications where traditional matrices such as alginate may fall short. The effect of environmental factors, such as temperature, pH, and substrate concentration, have also been studied on the hydrolysis rate and compared with the other immobilizing systems used for the same reaction, such as calcium alginate. Under the optimal conditions, penicillin G conversion reached 91.5% after 6 h and remained over 80% after 45 repeated cycles of 6 h each.

Multimedia

No record.

Cites

No record.

References

ISOLATION AND PURIFICATION OF PENICILLIN G ACYLASE OBTAINED FROM ESCHERICHIA COLI (NCIM-2400) AND IMMOBILISATION ON EUPERGIT C FOR THE PRODUCTION OF 6 AMINO PENICILLANIC ACID

Cite

APA: Copy

OTADY, M., VAZIRI, A., SEYF KORDI, A.A., & KHEYR ALOMOUM, A.. (2005). GUM TRAGACANTH GELS AS A NEW SUPPORTING MATRIX FOR IMMOBILIZATION OF WHOLE-CELL. IRANIAN JOURNAL OF CHEMISTRY AND CHEMICAL ENGINEERING (IJCCE), 24(4), 1-7. SID. https://sid.ir/paper/528984/en

Vancouver: Copy

OTADY M., VAZIRI A., SEYF KORDI A.A., KHEYR ALOMOUM A.. GUM TRAGACANTH GELS AS A NEW SUPPORTING MATRIX FOR IMMOBILIZATION OF WHOLE-CELL. IRANIAN JOURNAL OF CHEMISTRY AND CHEMICAL ENGINEERING (IJCCE)[Internet]. 2005;24(4):1-7. Available from: https://sid.ir/paper/528984/en

IEEE: Copy

M. OTADY, A. VAZIRI, A.A. SEYF KORDI, and A. KHEYR ALOMOUM, “GUM TRAGACANTH GELS AS A NEW SUPPORTING MATRIX FOR IMMOBILIZATION OF WHOLE-CELL,” IRANIAN JOURNAL OF CHEMISTRY AND CHEMICAL ENGINEERING (IJCCE), vol. 24, no. 4, pp. 1–7, 2005, [Online]. Available: https://sid.ir/paper/528984/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops