INFLUENCE OF HISTIDINE-HISTIDINE INTERACTION ON ACTIVITY, STABILITY AND STRUCTURE OF URICASE

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DASHTBAN MOGHADAM ELAHE; AKBARI NEDA; KHAJEH KHOSRO

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Journal Paper

Paper Information

Journal: MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS Year:2014 | Volume:3 | Issue:SUPPL. (1) Page(s): 119-119

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Title

INFLUENCE OF HISTIDINE-HISTIDINE INTERACTION ON ACTIVITY, STABILITY AND STRUCTURE OF URICASE

Author(s)

DASHTBAN MOGHADAM ELAHE | AKBARI NEDA | KHAJEH KHOSRO

Keywords

DRUG ENZYMES

URATE OXIDASE

THERMAL STABILITY

CATALYTIC EFFICIENCY

Abstract

Enzymes as drugs have two important features that distinguish them from all other types of drugs. First, enzymes often bind and act on their targets with great affinity and specificity. Second, enzymes are catalytic and convert multiple target molecules to the desired products. These two features make enzymes specific and potent drugs that can accomplish therapeutic biochemistry in the body that small molecules cannot. These characteristics have resulted in the development of many enzyme drugs for a wide range of disorders. URATE OXIDASE or uricase catalyzes oxidation of uric acid to alantoin which can be produced as a recombinant enzyme drug for treatment of hyperuricemia. The inherent poor stability of this enzyme is known to limit its efficacy, So that, stabilization of this enzyme is considered.There are a lot of factors that influence proteins stability; recent studies have shown that His-His interactions could have be an important role in stability and activity of proteins. For this purpose, we used site-directed mutagenesis (SDM) to substitute Gly 267 with His, Ala and Pro. Purified variants were biochemically characterized and compared with the wild type. Results revealed considerable changes in the CATALYTIC EFFICIENCY, THERMAL STABILITY and tertiary structures.

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APA: Copy

DASHTBAN MOGHADAM, ELAHE, AKBARI, NEDA, & KHAJEH, KHOSRO. (2014). INFLUENCE OF HISTIDINE-HISTIDINE INTERACTION ON ACTIVITY, STABILITY AND STRUCTURE OF URICASE. MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS, 3(SUPPL. (1)), 119-119. SID. https://sid.ir/paper/336115/en

Vancouver: Copy

DASHTBAN MOGHADAM ELAHE, AKBARI NEDA, KHAJEH KHOSRO. INFLUENCE OF HISTIDINE-HISTIDINE INTERACTION ON ACTIVITY, STABILITY AND STRUCTURE OF URICASE. MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS[Internet]. 2014;3(SUPPL. (1)):119-119. Available from: https://sid.ir/paper/336115/en

IEEE: Copy

ELAHE DASHTBAN MOGHADAM, NEDA AKBARI, and KHOSRO KHAJEH, “INFLUENCE OF HISTIDINE-HISTIDINE INTERACTION ON ACTIVITY, STABILITY AND STRUCTURE OF URICASE,” MOLECULAR BIOLOGY RESEARCH COMMUNICATIONS, vol. 3, no. SUPPL. (1), pp. 119–119, 2014, [Online]. Available: https://sid.ir/paper/336115/en

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