KLEBSIELLA PNEUMONIAE, Β-LACTAMASES, 16S RRNA METHYLASE, ARMA, BLACTX-M-15

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GOUDARZI HOSSEIN; AGHAMOHAMMAD SHADI; HASHEMI ALI

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Journal Paper

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Journal: Iranian Journal of Public Health Year:2014 | Volume:43 | Issue:SUPPLEMENT 2 Page(s): 86-86

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Title

KLEBSIELLA PNEUMONIAE, Β-LACTAMASES, 16S RRNA METHYLASE, ARMA, BLACTX-M-15

Author(s)

GOUDARZI HOSSEIN | AGHAMOHAMMAD SHADI | HASHEMI ALI

Keywords

ESCHERICHIA COLI

UTI

CHILDREN

ESBLS

Abstract

Background: The emergence of antibacterial resistance and ESBL-producing ESCHERICHIA COLI recovered from urinary tract infections (UTI) are known as important health problems in different regions, therefore therapeutic options for these infections are limited. The aim of this study was the detection of blaTEM, blaSHV and blaCTX-M genesamong ESCHERICHIA COLIstrains isolated from CHILDREN Medical Center hospital, Tehran, Iran.Methods: This study was conducted on 100 ESCHERICHIA COLI isolates from urine specimens of patients with UTI who referred to CHILDREN Medical Center, Tehran, Iran from November 2012 and July 2013. Antibiotic susceptibility test was performed by Kirby-Bauer disc diffusion method according to CLSI guidelines. The blaCTX-M, blaTEM and blaSHVgenes were detected by PCR and sequencing methods.Results: The resistance rate of isolates to Gentamicin, Imipenem, Nitrofurantoin, Cefotaxime, Ceftazidime, Amikacin, Cefepime, Piperacillin/Tazobactam, Cotrimoxazole, Cefixime andCephalothin, were 25 (25%), 0 (0%), 7 (7%), 51 (51%), 24 (24%), 2 (2%), 6 (6%), 2 (2%), 79 (79%), 36 (36%) and 43 (43%) respectively. In this study, imipenemwas more active than other antibiotics. The existence of blaTEM- 1 and blaCTX-M-15was detected in 69 (69%) and 74 (74%) of isolates respectively, while blaSHVgene was not detected.Conclusion: The prevalence of ESBL-producing E. coli detected in this study is of great concern and highlights the need of infection control measures including antibacterial management and prompt identification of ESBL-producing isolates.

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APA: Copy

GOUDARZI, HOSSEIN, AGHAMOHAMMAD, SHADI, & HASHEMI, ALI. (2014). KLEBSIELLA PNEUMONIAE, ?-LACTAMASES, 16S RRNA METHYLASE, ARMA, BLACTX-M-15. IRANIAN JOURNAL OF PUBLIC HEALTH, 43(SUPPLEMENT 2), 86-86. SID. https://sid.ir/paper/273375/en

Vancouver: Copy

GOUDARZI HOSSEIN, AGHAMOHAMMAD SHADI, HASHEMI ALI. KLEBSIELLA PNEUMONIAE, ?-LACTAMASES, 16S RRNA METHYLASE, ARMA, BLACTX-M-15. IRANIAN JOURNAL OF PUBLIC HEALTH[Internet]. 2014;43(SUPPLEMENT 2):86-86. Available from: https://sid.ir/paper/273375/en

IEEE: Copy

HOSSEIN GOUDARZI, SHADI AGHAMOHAMMAD, and ALI HASHEMI, “KLEBSIELLA PNEUMONIAE, ?-LACTAMASES, 16S RRNA METHYLASE, ARMA, BLACTX-M-15,” IRANIAN JOURNAL OF PUBLIC HEALTH, vol. 43, no. SUPPLEMENT 2, pp. 86–86, 2014, [Online]. Available: https://sid.ir/paper/273375/en

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