CLONING AND EXPRESSION OF LACCASE ENZYME FROM B. ANTHRACIS STRAIN QZA2

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ZAMANI P.; AMOOZEGAR M.A.; KHAJEH KH.

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Journal: JOURNAL OF MOLECULAR AND CELLULAR RESEARCHES Year:2015 | Volume:28 | Issue:1 Page(s): 54-64

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Title

CLONING AND EXPRESSION OF LACCASE ENZYME FROM B. ANTHRACIS STRAIN QZA2

Author(s)

ZAMANI P. | AMOOZEGAR M.A. | KHAJEH KH. | Issue Writer Certificate

Keywords

LACCASEQ2

MULTICOPPER OXIDASEQ2

CLONINGQ2

BACILLUS ANTHRACISQ1

Abstract

Laccases have many biotechnological applications due to their oxidation ability towards a wide range of phenolic compounds. In this study, the strain QZA2 isolated from Iran soils was used. This strain showed high similarity to Bacillus anthracis strain ATCC 14578 according to 16S rRNA gene sequence. Laccase gene was amplified by cloning primers. then PCR product cloned in the expression vector (pET21a) and transferred to BL21(DE3) strain of E. coli under the control phage T7 and sequence analysis carried out. The gene of the QZA2 has an open reading frame composed of 1656 bases, which encodes 551 amino acid residues and contain four histidine rich copper-binding domains. The laccase gene from QZA2 showed 16% similarity with CotA from B. subtilis and 57% similarity with multicopper oxidase B. halodurans. The expression was performed under microaerobic condition in order to obtain high amounts of soluble protein. Biochemical properties were investigated using common laccase substrates ABTS.

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APA: Copy

ZAMANI, P., AMOOZEGAR, M.A., & KHAJEH, KH.. (2015). CLONING AND EXPRESSION OF LACCASE ENZYME FROM B. ANTHRACIS STRAIN QZA2. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 28(1), 54-64. SID. https://sid.ir/paper/248489/en

Vancouver: Copy

ZAMANI P., AMOOZEGAR M.A., KHAJEH KH.. CLONING AND EXPRESSION OF LACCASE ENZYME FROM B. ANTHRACIS STRAIN QZA2. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2015;28(1):54-64. Available from: https://sid.ir/paper/248489/en

IEEE: Copy

P. ZAMANI, M.A. AMOOZEGAR, and KH. KHAJEH, “CLONING AND EXPRESSION OF LACCASE ENZYME FROM B. ANTHRACIS STRAIN QZA2,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 28, no. 1, pp. 54–64, 2015, [Online]. Available: https://sid.ir/paper/248489/en

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