BRANCHING AND ROOTING OF POLYGONUM BISTORTA IN VITRO

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KAVOUSI N.; ABBASPOUR H.; CHAICHI M.

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Paper Information

Journal: JOURNAL OF PLANT ENVIRONMENTAL PHYSIOLOGY Year:2013 | Volume:8 | Issue:2 (30) Page(s): 60-66

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Title

BRANCHING AND ROOTING OF POLYGONUM BISTORTA IN VITRO

Author(s)

KAVOUSI N. | ABBASPOUR H. | CHAICHI M. | Issue Writer Certificate

Keywords

BAQ2

BA+NAAQ2

POLYGONUM BISTORTQ2

IBAQ2

KIQ2

ROOTINGQ2

SHOOTSQ2

Abstract

In this study, the effect of plant growth regulators was investigated on shoot and root explants of Polygonium bistorta terminal buds. The experiment was conducted as a completely randomized design using 1, 2 and 3 mg/L KInetin, 0.1, 0.2 and 0.3 mg/L BA and combination of BA (0.2, 0.3 and 0.4 mg/L) + NAA (0.1 and 0.2 mg/L), in MS culture medium with three replications. The plantlets were planted using the terminal buds growing method in the MS medium under appropriate light and temperature conditions. After one month, the plantlets reached a sufficient growth and were ready for ROOTING. In the second experiment, the propagated plantlets were subjected to 0.5, 1, 1.5 and 2 mg/L IBA for ROOTING BAsed on a completely randomized design with 3 replications. After 45 days, the fully-rooted plantlets were transferred to a green house and planted in the vases and, thereafter, cultivation operations including irrigation, fertilization and spraying were carried out. Results showed that the use of growth regulators at different concentrations led to branch, root and leaf production in different sizes. 0.1 mg/L BA had the greatest effect on the production of shootings and leaves, while, the combination of BA+NAA had approximately the lowest effect. 0.5 and 1 mg/L IBA resulted in an increase in the root length, while 0.5 mg/L IBA affected the number of roots.

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APA: Copy

KAVOUSI, N., ABBASPOUR, H., & CHAICHI, M.. (2013). BRANCHING AND ROOTING OF POLYGONUM BISTORTA IN VITRO. JOURNAL OF PLANT ENVIRONMENTAL PHYSIOLOGY, 8(2 (30)), 60-66. SID. https://sid.ir/paper/185536/en

Vancouver: Copy

KAVOUSI N., ABBASPOUR H., CHAICHI M.. BRANCHING AND ROOTING OF POLYGONUM BISTORTA IN VITRO. JOURNAL OF PLANT ENVIRONMENTAL PHYSIOLOGY[Internet]. 2013;8(2 (30)):60-66. Available from: https://sid.ir/paper/185536/en

IEEE: Copy

N. KAVOUSI, H. ABBASPOUR, and M. CHAICHI, “BRANCHING AND ROOTING OF POLYGONUM BISTORTA IN VITRO,” JOURNAL OF PLANT ENVIRONMENTAL PHYSIOLOGY, vol. 8, no. 2 (30), pp. 60–66, 2013, [Online]. Available: https://sid.ir/paper/185536/en

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