Background: In this study, we isolated nickel resistance fungi and evaluated their potential for bioremediation application in acidic pH condition.Methods: Soil samples were collected from heavy metal contaminated parts of Iran. Fungal colonies were isolated and purified on potato dextrose agar (PDA), pH 5.7, amended with 400 mg/l of nickel. The plates were incubated at 28oC for 2 weeks. Purified isolates were tested for tolerance against 1000 mg/l of Ni in PDB, pH 2.5 with incubation at 28oC for 7 days. Selected strains were screened for ability to grow in PDB containing 1000 mg/l of Ni, pH 1, 1.5 and 2. For molecular characterization of the final selected isolate, genomic DNA extraction and subsequently, PCR amplification using nu-ssu-1536 and nu-ssu-817 primers was performed. PCR product was sequenced by Macrogen. The sequence was compared with those of other validated species using BLAST program.Results: Using Ni supplemented medium and cultivation conditions described before, a total of 120 fungal colonies were obtained from15 soil samples. Most of the isolates showed heavy growth on PDA plates. The entire isolates were preserved in University of Tehran Microorganisms Collection (UTMC). During the primary screening of the whole 120 purified isolates, 11 strains enabled to tolerate 1000 mg/l of Ni in acidic pH (2.5) in liquid culture. Among these 11 isolates, only 5 isolates grown in pH 2 (containing 1000 mg/l). No growth was seen in lower pH (1 and 1.5), with the same concentration of Ni. Finally, based on morphological characteristics and growth rate in presence of Ni, the strain UTMC 5002 was selected for phylogenic identification. Based on BLAST results, isolate UTMC 5002 belongs to Metarhizium anisopliae with 100% similarity. The nucleotide sequence data has been deposited at GenBank under accession numbers KM008603. This is the first report of Metarhizium sp. UTMC 5002 heavy metal removal and growth in acidic condition.Conclusion: Isolation and characterization of acidophilic and acid tolerant fungi, which are capable to remove heavy metals from contaminated solutions, is a new field of interest in Bioremediation technology. Current data provide a prerequisite for developing an efficient fungal Ni biosorbent using, Metarhizium sp. UTMC 5002.