Background. there are several methods for the bone grafting and improve osteogenesis in dental implant. one of the acceptable treatment for increase the volume of jaw bone is PRF. Nowdays reaserchers remove anti-coagulan can help them to improve bone grafting and named that I-PRF Methods. This results origin from some reaserches Results. Standard PRP and i-PRF (centrifuged at 700 rpm (60× g) for 3 min) were compared by assays for fibroblast biocompatibility, migration, adhesion, proliferation, as well as expression of platelet-derived growth factor (PDGF), transforming growth factor-β (TGF-β ), collagen1 (COL1) and fibronectin (FN). The results demonstrate that i-PRF induced significantly higher cell migration, as well as higher messenger RNA (mRNA) levels of PDGF, TGF-β , collagen1 and fibronectin when compared to PRP. Furthermore, collagen1 synthesis was highes Six Merino sheep received a total of 36 Brå nemark MKIII implants; three implants were placed supracrestally in each tibia with vertical exposure of four threads. Each implant received one of the three grafting options (MinerOss + PRF or MinerOss or PRF). The grafting materials were covered with a resorbable collagen membrane (Mem-Lok 30 × 40 mm, BioHorizons). Animals were sacrificed at 4 and 8 weeks, respectively, and specimens were prepared and collected for histologic analysis. Ground sections and decalcified sections were prepared in the i-PRF group. Conclusions. i-PRF demonstrated the ability to release higher concentrations of various growth factors and induced higher fibroblast migration and expression of PDGF, TGF-β , and collagen1.

Statement of the Problem: Currently, the reconstruction of bone defects with new platelet concentrates is considered a significant challenge in periodontics. Purpose: This study aimed to evaluate advanced-platelet rich fibrin (A-PRF) and leukocyte-and platelet rich fibrin’, s (L-PRF) effects on the proliferation and differentiation of MG-63 cells. Materials and Method: In this in vitro study, blood samples of five healthy non-smoking volunteers were collected and immediately centrifuged according to the two protocols of Choukroun and Ghanaati, without adding any anticoagulants, to prepare L-PRF and A-PRF. After freezing the clots for one hour, they were crushed and centrifuged once more. After culturing MG-63 cells, the effects of 20%, 10%, 1%, and 0. 5% concentrations of A-PRF and L-PRF extracts on cell proliferation and mineralization were evaluated by methyl thiazolyl tetrazolium (MTT) assay and Alizarin Red staining, respectively. Results: Generally, survival and proliferation in the L-PRF group at both time intervals were higher than the A-PRF group and increased with increasing the extract concentration. However, in the A-PRF group, there were no significant differences between the different concentrations, and only the number of cells increased over time. After three days, in the study on mineralization, nodule formation was observed only in the positive control group (osteogenic). In seven days, mineralized nodules were formed in all groups with different concentrations of A-PRF, but not in any of the L-PRF groups. Conclusion: According to the results, L-PRF increased proliferation, and A-PRF exerted a positive effect on the differentiation of MG-63 cells.

Fibrin-rich plasma is a material that is easy to make, requires only the patient's blood, thus nullifies the chances of contamination and reduces the risk of infection. Its aspects of hemostasis and support of the immune system contribute to its satisfactory result. L-PRF has wide applicability, from dentistry to medicine, with excellent results in short and medium terms. Several studies have shown safety in its use for maxillofacial application. This study aims to make a brief review of the literature in the last 15 years regarding the use of L-PRF and its applicability in dentistry in general.

Introduction: Tendon injury is an important clinical issue for orthopedic surgeons. Tendon healing is a time-consuming process. This study aimed to investigate the effect of autologous Leukocyte-platelet-rich fibrin (L-PRF) on the healing of the superficial digital flexor tendon in a rabbit model. Method: Ten healthy adult rabbits of mixed breed were randomly divided into Treatment and Control groups. After cutting the superficial digital flexor tendon and suturing it, the autologous L-PRF membrane was applied locally in the Treated group. The tendon was sutured with the Bunnell-Mayer pattern with no material implantation in the Control group. Five animals from each group were euthanized, and their tendons were collected for histopathological evaluation on the 30th day post-surgery. Result: In morphological examination, a smaller tendon diameter and more adhesion were seen in the Treated group. There were significant differences in the total number of cells, cell maturation, the ratio of cellularity to matrix, and the alignment and order of cells between the Treated and Control groups at the histopathological level. The Treated group showed superiority over the Control group in tissue order, collagen fiber crimp pattern, inflammation, and vascularity; however, these differences were not significant. Conclusion:  This study found that L-PRF has a beneficial effect on tendon healing. Due to the short treatment period, additional research and evaluations are necessary in this field.