Background and Aim: In humans, many diseases are associated with the accumulation of free radicals. Antioxidants can scavenge free radicals and minimize their impact. Therefore, the search for naturally occurring antioxidants of plant origin is imperative. Methods: Here, we aimed to investigate the antioxidant and free radical SCAVENGING properties of methanolic extracts from Plantago major, Asperugo procumbens, Fumaria paviflora. Methanolic extract of P. major, A. procumbens, F. paviflora leaf is a potential source of natural antioxidants and serves as an effective free radical scavenger and/or inhibitor. Hence, of P. major, A. procumbens, F. paviflora might be a good plant-based pharmaceutical product for several diseases caused by free radicals. Results: In this experiment, we examined different parts (leaf) of A. procumbens and found that methanolic extract of A. procumbens leaf, which contains large amounts of phenolic and flavonoid compounds, exhibited the highest antioxidant and free radical SCAVENGING. A positive correlation (P-value < 0. 005) was observed between phenolic content and free radical (DPPH) SCAVENGING efficiencies. Conclusion: Methanolic extract of A. procumbens leaf is a potential source of natural antioxidants and serves as an effective free radical scavenger and/or inhibitor. Hence, A. procumbens might be a good plant-based pharmaceutical product for several diseases caused by free radicals.

Objective(s): The unfavorable physicochemical properties of well recognized antioxidant phytoactive sesamol limits its oral bioavailability as well as potential application as an antioxidant drug. The aim of the study is to design and evaluate sesamol encapsulated hyaluronic acid anchored phospholipid nanovesicles to enhance its antioxidant potential. Methods: Drug encapsulated hyalurosomes were prepared using thin film hydration method and evaluated for particle diameter, physical stability, drug encapsulation efficiency, sesamol release behavior in vitro and DPPH radical SCAVENGING assay. Results: The selected method was found to be effective for fabrication of phospholipid nanovesicles with particle diameter 200 ±,10. 173 nm and zeta potential-29. 8 ±,4. 16 mV. The drug loaded hyalurosomes revealed significantly better radical SCAVENGING potential compared to free sesamol and unloaded hyalurosomes. Conclusions: Hyaluronic acid functionalized phospholipid nanovesicles is novel phospholipid based carrier for delivery of phytoactives. Thus formulated phospholipid based system could be acceptable system for delivery sesamol with improved antioxidant potential.

Reactions of free radicals and reactive oxygen species (ROS) with biological molecules in vivo play an important physiological role in many diseases.2, 2-diphenyl-1-picrylhydrazyl (DPPH) is a stable free radical and often used as a routine reagent to evaluate the antioxidant capacity of an antioxidant. This study was undertaken to investigate the free radical-SCAVENGING and antioxidant activities of Zinc, Vitamin C and mixture of them. UV-Vis spectrophotometry method was used to evaluate the ability of Zinc, Vitamin C and mixture of them to scavenge DPPH radical. The kinetic parameters such as rate constant and activation energy in experimental conditions were calculated. The rate constants of the H atom abstraction by DPPH (1 k), in the presence of Zinc, Vitamin C and mixture of them were obtained (0.4209, 2.092 and 1.82 min-1) respectively, under pseudo-first-order conditions at 25oC.