Angiogenesis is an important biological process. The VEGF and VEGFR pathway is one of most important pathways, which is involved in this process. Recent studies have identified several signaling pathways and regulatory factors governing angiogenesis in vertebrates. However, understanding the molecular details of these interactions has been complicated by extensive gene duplication events in vertebrate. To circumvent this genetic redundancy, we have focused on VEGFR in basal chordate (urochordates (Sea squirt)).By using rapid amplification of cDNA from total RNA with primers specific to tyrosine kinase domain and further extension of cDNA, we isolated a part of sea squirt cDNA. We found the sequence is highly homologous to human VEGFR1.The sequence also shows a higher homology with 3 human VEGFRs than PDGFR suggests that this sequence encodes for 7-Ig VEGFR-like molecule in Sea squirt. Therefore, we tentatively designated it as S.sqVEGFR. We also extracted total RNA from different Sea squirt tissues (Muscle, Intestine, Stomach, Pharynx, Gill). By using PCR of reverse-transcribed total RNA extracted from different tissues, we showed the expression of sq. VEGFR in this animal was significantly different among the Sea squirt tissues. So that, RT-PCR showed S.sq VEGFR has the most strong expression in stomach which is the closest organ to the heart and weak expression in the other remote organs. Western blot and histology data also showed the same results. Therefore, analysis our data revealed there is high correlation between the concentration of blood cells and vessels and the level of expression of S.sq VEGFR. Suggesting that expression of S.sq VEGFR is related to blood cells and vessels. These findings may shed more light on the function of VEGFR in angiogenesis.