Seed protein usually is not sensitive to environmental fluctuations; its banding pattern is very stable which advocated for cultivars identification purpose in crop. This study was planned to study effect of water deficit on protein content and protein banding pattern of wheat genotypes. In this study five wheat genotypes were planted in early November, growing season of 2008-2009. The experiment was carried out in a factorial design with three replications. Two water use levels (with and without irrigation) and five genotype of wheat (SABALAN/4/VRZ/3/OR F1.148/TDL//BLO, HAMAM-4, KAUZ' S' /MACHETE, Atila2/PBW65 and M-79-7) factorialed in plots as random. proteins profiling of samples was performed using SDS- polyacryl amide gels. The results showed that, No effects Non irrigated treatments on protein banding patterns. Also, results indicated that not obvious any new band and not deleted any bands. Among the genotypes treatments HAMAM-4 genotype in Non irrigated condition had the highest seed protein and the KAUZ' S' /MACHETE genotype in irrigated condition had the lowest seed protein.