IN ORDER TO STUDY THE FUNCTION OF GEMINI SURFACTANT AS CELL MEMBRANE MIMETIC IN THE IN VITRO STUDIES, INTERACTION TYPES OF THIS LIPID COMPOSED OF NHEXADECYLE TAILS, DIMETHYL AMMONIUM HEADS AND (CH2)5 CARBON LINKER (GSP) WITH AN ANIONIC PROTEIN, RIBONUCLEASE SA (RNASE SA), WAS INVESTIGATED BY MOLECULAR DYNAMICS (MD) SIMULATIONS. ...

PURPOSE: THIS WORK WAS PREPARED A NIOSOMAL FORMULATION OF SILIBININ FOR IMPROVEMENT OF DRUG EFFICIENCY AND ALSO REDUCING THE REQUIRE DOSE AND ITS SIDE EFFECTS. NIOSOMES ARE AN ALREADY ESTABLISHED ENCAPSULATION TECHNOLOGY IN DIFFERENT AREAS INCLUDING FOOD, BIOTECHNOLOGY, COSMETICS AND PHARMACEUTICS. THIS STUDY AIMED TO PREPARE AND INSPECT THE GENERAL PROPERTIES OF NIOSOMAL SILIBININ AS A NEW DRUG DELIVERY SYSTEM [1].MATERIALS AND METHODS: NIOSOMES WAS PROVIDED THROUGH REVERSE PHASE EVAPORATION METHOD. IN THIS METHOD, DIFFERENT PROPORTIONS OF SPAN 20, CHOLESTEROL, MPEG-2000 AND SILIBININ WERE MIXED TOGETHER IN DICETYL PHOSPHATE AS SOLVENT. AFTER SEPARATION OF THE SOLVENT USING ROTARY EVAPORATOR, THE GELLOSE FILM WAS HYDRATED IN BUFFER PHOSPHATE SALINE, SONICATED AND THEN HOMOGENIZED. ENTRAPMENT EFFICIENCY, VESICLES SIZE, STABILITY AND DRUG RELEASE PATTERN WAS STUDIED BY RELATED METHODS [2-4].RESULTS AND DISCUSSION: THE MEAN DIAMETER OF NIOSOMAL SILIBININ WAS OBTAINED 178.4±4 BY ZETA SIZER DEVICE. THEIR ENCAPSULATION EFFICIENCY WAS MEASURED BY INDIRECT METHOD USING HIGH SPEED CENTRIFUGE AND WAS OBTAINED 94±2%. RELEASE STUDY OF NIOSOMAL SILIBININ WAS DONE BY DIALYSIS METHOD AND THE RESULTS SHOW THAT THE NEW FORMULATION IS STABLE AT 4°C AFTER 4 WEEKS. STABILITY AND THE RESULTANT EASE OF STORAGE OF NIOSOMES HAVE LED TO THE EXPLOITATION OF THESE CARRIERS AS ALTERNATIVES TO OTHER NANOENCAPSULATION TECHNOLOGIES.