This study was conducted to understand the macroscopic and microscopic structure of the fibrous tunic of 20 healthy adult ostrich eyes. First, the fibrous tunic of the eye was anatomically examined in terms of appearance, dimensions, location and structure. Then prepared histological slides were studied microscopically after staining by H&E, Verhoeff, Van Geison and P.A.S. The results showed that in the ostrich ovally round cornea, the average corneal length (lateral-medial) and width (dorsal-ventral) were respectively measured as 2.58±0.05 and 2.28±0.04 centimeters. The corneal protrusion was 0.92±0.02. The thickest part of sclera was in the posterior portion of the eyeball, in optic nerve entrance. The whole thickness of cornea and its epithelium were measured 850 µm and 65 µm respectively. Anterior surface of cornea was nonkeratinized stratified squamous epithelium. There was Bowman's membrane with 7.5 mm thickness and positive P.A.S. reaction Descemet's membrane with 7.65 mm thicknesses. 450 mm-ticked sclera in ostrich had frequent collagen fibers which supported by hyaline cartilage with 217.8 mm thickness. Moreover, there were no elastic fibers in cornea and a little in sclera. It concluded that although there were little differences in dimensions and microscopic studies, the fibrous tunic of ostrich was similar to other birds but different from mammals.