Nutrition as an environmental factor has a special place in the growth, main and sub branches number ofRosa damascene Mill. and these morphological traits affected yield and flower number of the plant. So, this experiment was conducted in the Research Institute of Forest and Rangelands, Karaj, Iran, to evaluate the effect of macro- and micronutrients on the plant growth. A split plot design was conducted based on randomized complete block design with three replications. The main factor was combination of fertilizer in 5 levels: 1) N0, P0 & K0 kg/ha+manure 0 ton/ha, 2) N40, P40 & K0kg/ha+manure 15 ton/ha, 3) N40, P40 & K40kg/ha+manure 15ton/ha, 4) N80, P80 & K40 kg/ha+manure 30ton/ha & 5) N120, P120 & K80kg/ha+manure 40ton/ha. The sub factor was micro nutrients intake of iron chelate in 3 levels (0, 8 and 12 g). The analysis of variance showed that the main and sub factor effect were significantly different for all of measured traits (a≤0.01). Results indicated that the highest main branch (66.35cm) and sub branch (15.96 cm) growth belonged to treatment 2 and micronutrients intake of 0 g, respectively. Treatment 4×8g showed the highest main branches number (49.66 n/plant). The highest sub branch number with average value of 26.66n/plant was obtained in treatment 4×0g.According to positive correlation between bush height with yield and flower number per bush, treatment 2×12g could be used for increasing quality and quantity flower yield in R. damascena.