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مرکز اطلاعات علمی SID1
مرکز اطلاعات علمی SID
اسکوپوس
مرکز اطلاعات علمی SID
ریسرچگیت
strs
اطلاعات دوره: 
  • سال: 

    2017
  • دوره: 

    0
  • شماره: 

    1
تعامل: 
  • بازدید: 

    1575
  • دانلود: 

    0
چکیده: 

HYDATIDOSIS, CAUSED BY echinococcus granulosus IS ONE OF THE MOST IMPORTANT ZOONOTIC DISEASES, THROUGHOUT MOST PARTS OF THE WORLD. HYDATIDOSIS IS ENDEMIC IN IRAN AND RESPONSIBLE FOR APPROXIMATELY 1% OF ADMISSION TO SURGICAL WARDS. …

آمار یکساله:  

بازدید 1575

دانلود 0
نویسندگان: 

نشریه: 

FRONTIERS IN MEDICINE

اطلاعات دوره: 
  • سال: 

    2018
  • دوره: 

    5
  • شماره: 

    -
  • صفحات: 

    0-0
تعامل: 
  • استنادات: 

    315
  • بازدید: 

    6122
  • دانلود: 

    9195
کلیدواژه: 
چکیده: 

آمار یکساله:  

بازدید 6122

دانلود 9195 استناد 315 مرجع 0
اطلاعات دوره: 
  • سال: 

    2020
  • دوره: 

    49
  • شماره: 

    1
  • صفحات: 

    181-185
تعامل: 
  • استنادات: 

    0
  • بازدید: 

    13427
  • دانلود: 

    8819
چکیده: 

Background: The main objective of the current study was to investigate on the cryopreservation of protoscoleces of echinococcus granulosus, a causative agent of cystic hydatidosis in man. Methods: This study was conducted on isolated protoscoleces from hydatid cysts infected livers collected from slaughterhouse of Tehran, Iran in 2016. Viability of protoscoleces was evaluated by dye test. Cryopreservation of isolated protoscoleces in the presence of Dimethylsulfoxide (DMSO) and glycerol using a three-step cooling protocol involving an initial period at-20 ° C,-80 ° C and liquid nitrogen was performed. Results: The mean viability rate of 10% DMSO and 15% glycerol were 9% and 8% respectively. The protoscoleces of echinococcus granulosus have been successfully thawed and recovered after 6 months storage in liquid nitrogen. Conclusion: Cryopreservation method needs to be improved for each species of helminthes and can be useful for other immunological and laboratorial studies.

آمار یکساله:  

بازدید 13427

دانلود 8819 استناد 0 مرجع 0
گارگاه ها آموزشی
اطلاعات دوره: 
  • سال: 

    1381
  • دوره: 

    15
  • شماره: 

    1 (پی آیند 54) در امور دام و آبزیان
  • صفحات: 

    50-52
تعامل: 
  • استنادات: 

    4
  • بازدید: 

    672
  • دانلود: 

    87
چکیده: 

هدف از این مطالعه تعیین وضعیت آلودگی سگ سانان به فرم بالغ E. granulosus و تعیین وضعیت آلودگی دامهای ذبح شده به کیست هیداتیک در کشتارگاههای استان کردستان بوده است. علاوه بر این، میزان باروری کیستهای جدا شده از دامها و اندامهای مختلف آنها و همچنین میزان زنده بودن پروتواکسولکس کیست دامها و اندامهای مختلف مورد ارزیابی قرار گرفت. بطور کلی از مجموع 35 قلاده سگ، 4 قلاده (11.43%) به E. granulosus آلوده بوده اند. در سه قلاده روباه قرمز انگل E. granulosus مشاهده نشد. در کشتارگاه سنندج استان کردستان، از مجموع 19425 راس گوسفند تحت مطالعه 5.43%، از مجموع 5938 راس بز، 3.06% و از مجموع 11883 رای گاو 9.49% به کیست هیداتیک آلوده بوده اند. در گوسفندان آلوده 75.35% آلودگی در ریه ها، 10.52% در کبد و 13.93% به صورت آلودگی توام و در بزهای آلوده 80.76% آلودگی در ریه ها، 6.91% در کبدها و 12.08% به صورت آلودگی توام و در گاوهای آلوده، 79.24% آلودگی در ریه ها، 5.49% در کبدها و 13.12% به صورت آلودگی توام بوده است. 23.82% کیستهای گوسفندان، 30.95% کیستهای بزها و 2.52% کیستهای گاوها دارای کیستهای بارور بوده و میانگین درصد زنده بودن پروتواسکولکس ها در کیستهای بارور دامهای تحت مطالعه اکثرا بیش از 70% بوده است.

آمار یکساله:  

بازدید 672

دانلود 87 استناد 4 مرجع 2
نویسندگان: 

HASHEMI TABAR GH.R. | RAZMI GH.R. | NAGHIBI A.

اطلاعات دوره: 
  • سال: 

    2006
  • دوره: 

    7
  • شماره: 

    3 (16)
  • صفحات: 

    56-59
تعامل: 
  • استنادات: 

    0
  • بازدید: 

    12993
  • دانلود: 

    7842
چکیده: 

We conducted this study to determine the level of immunity after vaccination of lambs with whole body echinococcus granulosus. To do so, 200 mature E. granulosus parasites, which were kept in 10% formaline for 8 months, were obtained from the Department of Parasitology, School of Veterinary Medicine, Ferdowsi University of Mashhad. The soluble protein of the parasite was prepared. The sample was homogenized in a blender, sonicated on ice and then centrifugated for 15 min at 10,000 g. Final yield was kept at -20ºC until used. Eight 4–6-month-old lambs of mixed sex, were divided into 2 equal groups; each lamb in the test group was vaccinated subcutaneously in the neck with 2 ml of the vaccine (1 mg of whole body of E. granulosus protein dissolved in 1 ml of PBS plus 1 ml of Freund’s complete adjuvant (FCA)). The control lambs were vaccinated only with adjuvant in PBS. Lambs were re-vaccinated four weeks after the first vaccination with the same preparation except that FCA was replaced by Freund’s incomplete adjuvant (FIA). Three weeks later, each lamb was administered a challenge infection dose of 2000 protoscolices intraperitoneally and 10 adult E. granulosus. After 7 months, all lambs were killed and examined for hydatid cysts. We found two cysts in the liver and and one in the lung of only one of the vaccinated lambs. The number of cysts in vaccinated lambs were significantly lower than that in the control group (P<0.001). This means that the protective immunity in lambs with whole body of E. granulosus was approximately 90%.    

آمار یکساله:  

بازدید 12993

دانلود 7842 استناد 0 مرجع 0
نویسندگان: 

BUTTENSCHOEN K. | CARLI BUTTENSCHOEN D.

اطلاعات دوره: 
  • سال: 

    2003
  • دوره: 

    388
  • شماره: 

    4
  • صفحات: 

    218-230
تعامل: 
  • استنادات: 

    360
  • بازدید: 

    5974
  • دانلود: 

    12462
کلیدواژه: 
چکیده: 

آمار یکساله:  

بازدید 5974

دانلود 12462 استناد 360 مرجع 0
strs
نویسنده: 

HASHEMI TABAR GH.R. | RAZMI GH.R. | NAGHIBI A.

اطلاعات دوره: 
  • سال: 

    2005
  • دوره: 

    0
  • شماره: 

    4
تعامل: 
  • بازدید: 

    3990
  • دانلود: 

    0
چکیده: 

In the present study, two hundred mature echinococcus granulosus, which were kept in 10% formaline for 8 months, obtained from parasitology Depatrment, School of Veterniary Medicine, Ferdowsi University of Mashhad. Protein soluble of parasite was prepared. The sample hemogenized in a blender, sonicated on ice and then centrifugated for 15min at 10000 g. Final yield was kept at -20oC until used. Eight Lambs 4-6 months of age of mixed sex were divided into 2 groups of 4 lambs. Each lamb in test group was vaccinted subcutaneously in the neck with a 2 ml dose of vaccine [1 mg of whole body of E. granulosus protein dissolved in 1 ml of PBS plus 1 ml of Freund’s complete adjuvant (FCA)]. Control lambs were vaccinated with adjuvant in PBS. Lambs were re-vaccinated four weeks after the first vaccination with the same preparation except that FCA was replaced by Freund’s incomplete adjuvant (FIA). Three weeks after the second vaccination, each lamb received a challenge infection with 2000 protoscolices intrapritoneally and 10 adult E. granulosus. All sheep were killed after 7 months and examined for hydatid cysts. Results showed that, in comparison to control lambs, olny in one of the vaccinated lamb two and one cyst were observed in liver and lung respectively. The number of cysts in vaccinted lambs were significantly lower than in control group (P<0.001). This result indicated that the protective immunity in sheep with whole body of E. granulosus was approximately 90%.

آمار یکساله:  

بازدید 3990

دانلود 0
نویسندگان: 

ERGIN S. | SARIBAS S. | YUKSEL P.

اطلاعات دوره: 
  • سال: 

    2010
  • دوره: 

    4
  • شماره: 

    7
  • صفحات: 

    551-555
تعامل: 
  • استنادات: 

    315
  • بازدید: 

    4577
  • دانلود: 

    9195
کلیدواژه: 
چکیده: 

آمار یکساله:  

بازدید 4577

دانلود 9195 استناد 315 مرجع 0
نویسندگان: 

HASHEMI TABAR GH.R. | RAZMI GH.R. | NAGHIBI A.A.GH.

اطلاعات دوره: 
  • سال: 

    2006
  • دوره: 

    10
  • شماره: 

    1
  • صفحات: 

    51-55
تعامل: 
  • استنادات: 

    0
  • بازدید: 

    21964
  • دانلود: 

    23116
چکیده: 

Despite the establishment of extensive and successful control programmes in some countries or regions, echinococcus granulosus still has a very wide geographical distribution. Mature E. granulosus (n = 120) obtained from Parasitology Department, School of Veterinary Medicine, Ferdowsi University of Mashhad (Iran). Soluble protein of whole body of parasite was prepared by freeze-thawing in liquid nitrogen and at 42oC for three times. The sample was homogenized in a blender, sonicated at 110 V, 170 W for 3 times for 15 s each and then centrifuged at 10,000 xg for 15 min. Final yield was kept in -20oC. Ten mice were randomly divided into 2 groups of 5. Each mouse in test group was vaccinated subcutaneously with 100 µg (100 µl) of whole body of E. granulosus plus 100 µl of Freund"s complete adjuvant (FCA), respectively. Mice in control group were vaccinated with adjuvant in PBS. Second vaccination was conducted after four weeks with the same preparation except that FCA was replaced by Freund"s incomplete adjuvant (FIA). Three weeks after the second vaccination, each mouse was challenged with 2000 protoscolices, intraperitoneally. Mouse autopsy was carried out eight months post challenge. Our results show that none of the vaccinated mice with the whole body of E. granulosus had cysts that indicate 100% protective immunity.

آمار یکساله:  

بازدید 21964

دانلود 23116 استناد 0 مرجع 0
اطلاعات دوره: 
  • سال: 

    2018
  • دوره: 

    13
  • شماره: 

    3
  • صفحات: 

    423-429
تعامل: 
  • استنادات: 

    0
  • بازدید: 

    15851
  • دانلود: 

    15809
چکیده: 

Background: Cystic echinococcosis is a zoonotic infection and considered as a major economic and public health concern worldwide. This research was conducted to determine genotypic characteristics of livestock and human hydatid cyst isolates from Hamadan area, western Iran. Methods: Sampling was conducted in Hamadan industrial slaughterhouse and Beast Hospital of Hamadan City, western Iran, from 2015 to 2016. Overall, 74 livestock isolates including 69 sheep, 3 cattle and 2 goats and 9 human hydatid cysts were genotyped by PCR amplification of the rDNA ITS1 region and followed restriction fragment length polymorphism (RFLP) analysis with four restriction endonuclease enzymes, RsaI, HpaII, AluI, and TaqΙ , and sequencing. Results: The PCR amplicon size of each isolate was approximately 1 kb which was the same with that of sheep strain. According to the RFLP patterns, the isolates belonged to a single species, E. granulosus sensu stricto (G1– G3 complex). Furthermore, sequencing of representative amplicons confirmed that the RFLP-genotyped isolates corresponded to E. granulosus sensu stricto. Conclusion: E. granulosus sensu stricto is the prevailing species of E. granulosus sensu lato in the region and pointed out the importance of sheep/dog cycle in human transmission.

آمار یکساله:  

بازدید 15851

دانلود 15809 استناد 0 مرجع 0
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