In order to evaluate the residual amount of nitrogen, phosphorous and potassium in soil in response to different rotations and conventional and ecological cropping systems, an experiment with split-plot design based on complete randomized block design with 3 replications was conducted for 6 years. Main plots were assigned to different rotations included, wheat-wheat, and corn-wheat and sugar beet-wheat. Sub plots were assigned to different cropping systems included high-input, medium-input, low-input, integrated (with moderate input) and organic (without any chemical input). Results showed that the amount of macronutrients in surface soil layer was not consistent. The amount of residual N in soil was reduced, but the amount of residual P and K were increased in 6 years. Also soil analysis showed that the residual amount of soil minerals in organic and integrated systems was more than conventional system. The residual amount of macronutrients was affected significantly by different rotations. The electrical conductivity of saturated extract in integrated system was the highest level and high-input, medium-input and low-input systems were lower than integrated system respectively, and the organic system was the lowest one