The species of Lactobacillus are widely present in many natural environments that are important in fermented processes. Among these species some belong to the lactic acid bacteria group that are useful in fermented food. One of the species of lactobacilli group such as L. plantarum provides health benefits, but their identification is one of the main issue. In order to proceed with this project, conventional culture method and pcr primers have been employed to identify L. plantarum. According to the conventional culture method, colonies were Gram-positive and catalase-negative in the form of coccoid-rod, short, long, thin and fine. Under the pcr conditions, L. plantarum and L. brevis strains were detected in only one sample at 25oC. In chemical technique, L. plantarum was separated from L. brevis in four cultures medium with 4-7% NaCl where L. brevis became inactive. The isolated L. plantarum that was used in the fermented cucumbers prevented the growth and development of pathogenic organisms.