In order to study the possibility of the cultural control of chilo suppresssalis Walker by means of crop rotation, two series of experiments were conducted at Amol research station during 200 I - 2003. Experimental design was RCBD based of factorial for both experiments. In the first experiment, the treatments were pea, faba, lettuce, garlic, and fallow control which population density and dynamics of overwintering larvae were studied on the crops. For density measurement of overwintering larvae, 5 samples (0.5 x 0.5 m2) of each 50 m2 plots were randomly taken and the alive and dead larvae in rice stems were counted. The second experiment was studying the infestation of two rice varieties including, Tarom and Fajr to the larvae after harvesting the alternative crops. to do this, each 50 m2 plot was divided to 2 parts and then the two rice varieties including, Tarom and Fajr were cultivated in the same plots. The results of the first experiment indicated that among a sampling steps. the number of larvae was maximum and minimum in the first and last steps, respectively. Also, the results of the second experiment indicated that Tarom variety had the higher amount of dead hearts and white heads than the Fajr. Results of studies on the rice infestation based on leaf sheath infestation, dead hearts, and white heads indicated that Tarom had the highest amount of dead hearts and white heads compared to Fajr. The effect of year on the dead larvae was not significant. Study of the effect of different sampling steps on the live and dead larvae indicated that the steps 3, 4, 5 and the last step had the highest and the lowest number, respectively. The results indicated that there was not significant differences between the varieties on the base of leaf sheath infestation, dead hearts, and white heads in 2002 and 2003. The results of this research indicated that, cultural control with cultivating of alternative crops has efficient role in control of C. suppressalis.