Background: Fatty streaks are the primary lesions to form atherosclerosis. Lipid per oxidation by free- radicals, plays an important role in plaque formation. VITAMIN E, as a lipid in soluble VITAMIN, is an important antioxidant and may prevent or delay the coronary heart disease by limiting LDL oxidation. Our goal was to evaluate the effect of VITAMIN E and iron on blood serum lipids in male rabbits fed high – cholestrol diet.Method: Thirty white male rabbits were weighed and blood serum samples were taken for analysis of serum lipoproteins. They were randomly divided in to 5 groups each Containing 6 rabbits for 42 days. Group1 was given normal diet, Group2 fed with high cholesterol (%2) diet, Group3fed with high cholesterol diet with iron (50 kg/mg), Group4 fed with high cholesterol diet with VITAMIN E (50 kg/mg) , Group5 fed with high cholesterol diet with iron (50 kg/mg) and VITAMIN E (50 kg/mg). These groups were again weighed and blood samples were taken for analysis of serum lipoproteins after 42 days.Results: Significant difference in cholesterol, LDL, HDL, TG, VLDL, were seen before and after the experiment in all 5 groups, (p<0/000).Conclusion: The significant difference was observed between all groups in relation to the effect of iron and VITAMIN E on lipid metabolism. While VITAMIN E has a protective role in atherosclerosis, it seems that iron has a provocation role in serum lipids.