Background: Zinc has important effects on structural and functional activities of many proteins and enzymes, specially regulation of immune system. Objective: This study was carried out to examine the in vitro effects of different concentration ofzinc on viability and morphology of RAJI CELL line. Methods: In this study the CELL line was exposed to different concentration of zinc(IOnM to 500µM)followedby incubation (37 c, 5%C02) at various time points(I2 to 72 hrs). The CELLs were then evaluated with trypan blue exclusion dye, and Wright-Gimsa staining. Findings: The results showed almost different responses to different amount of zinc by the RAJI CELLs. less than 100J.lMat different incubation time points had no effects on CELL line when compared to the controls. Higher concentrations of zinc (>100µM)viability diminished to 70% at ]2 hrs and less than 50% at 24 hrs of incubation times. Conclusion: We conclude that Zn has dose-dependent cytotoxic effect on RAJI CELLs and probably application for immune-modulation.

Background: Zinc has important effects on structural and functional activities of many proteins and enzymes, specially regulation of immune system. Objective: This study was carried out to examine the in vitro effects of different concentration of zinc on viability and morphology of RAJI CELL line. Methods: In this study the CELL line was exposed to different concentration of zinc(10nM to 500µM)followed by incubation (37 c, 5%Co2) at various time points(12 to 72 hrs). The CELLs were then evaluated with trypan blue exclusion dye , and Wright-Gimsa staining. Findings: The results showed almost different responses to different amount of zinc by the RAJI CELLs. less than 100µM at different incubation time points had no effects on CELL line when compared to the controls. Higher concentrations of zinc (>100µM) viability diminished to 70% at 12 hrs and less than 50% at 24 hrs of incubation times . Conclusion: We conclude that Zn has dose-dependent cytotoxic effect on RAJI CELLs and probably application for immune-modulation.

Introduction: Since 1960 and description of the Philadelphia chromosome, the association of specific structural and numerical chromosome abnormalities with certain types of malignancies has been appreciated. Therefore, the study of lymphoma CELLs lines may provide useful information about the cytogenetic evolution taking place during the establishment of the CELL lines. Methods: The CELL lines were cultured, harvested, resuspended in hypotonic solution and fixed. After trypsin treatment, the slides were stained using Leishman's method. The observed chromosomal abnormalities were then reported using ISCN 1995. In some cases, results of G-bandings were confirmed by FISH. In these cases mostly PCR amplified band-specific fluorescent-labeled probes, and for some abnormalities chromosome arm painting (CAP) probes were used. Results: Overall, analysis of CELL lines demonstrated that the chromosome number of the majority of the CELLs was 46 in Daudi and RAJI; this number for 45 for Namalwa. The most common chromosome abnormality detected in all CELLs analyzed was t (8:14) with classical breakpoints (q24; q32), but many other abnormalities were also detected, such as additional material on the short arm of chromosome 11 (Daudi), additional material on the q35 of ch 4 (RAJI) and an HSR attached to the long arm of one of the ch 1q (Namalwa) among others. Discussion: Our results demonstrated some well characterized chromosome abnormalities and also some variations in both the numerical and structural chromosomal abnormalities from those reported in other studies. Some of these chromosome abnormalities also have reported from Burkitt's lymphoma patents. Therefore characterizing these abnormalities might be of great importance in understanding the progression of the disease.  

Background: Zinc has significant effects on structural and functional activities of many proteins and enzymes involved in biological activities, especially the regulation of immune-system.Symptoms of zinc toxicity include nausea/vomiting, fever, cough, diarrhea, fatigue, neuropathy, and dehydration. Further signs include growth retardation, altered iron function, anemia, copper deficiency, decreased immune function, decreased HDL (high density lipoprotein), increased LDL (low density lipoprotein), and increased HgbA1C.This study was carried out to examine the invitro effects of different concentrations of zinc on viability and death of T-lymphoid (RAJI) CELL line.Methods: In this study, the CELL line was exposed to different concentrations of zinc (10nanoM to 500microM) followed by incubation (37° C, 5% CO2) at various time points (12 to 72 h). The CELLs were, then, evaluated using trypan blue exclusion dye, MTT assay (mitochondorial thiazol tetrazolium), and light microscopy.Results: The results of this study showed almost different responses to different amounts of zinc in the T CELL line (RAJI). Zinc concentrations below 100mM at different incubation time points had little or no effects on the CELL line compared to the controls. Higher concentrations of zinc viability (>10mM) diminished to 70% at 12 hour and less than 50% at 24 to 72 hours of incubation.Conclusion: It can be concluded that zinc has a dose-dependent cytotoxicity effect on RAJI CELLs.

Background: Zinc has important effects on human health, especially on structural and functional activities of the immune system. This study was carried out to examine the in vitro cytotoxic effects of zinc on RAJI CELL line. Materials and Methods: The CELL line was exposed to different concentrations of zinc followed by incubation (37°C, 5% CO2) at various time points (12 to 72 hrs).Viability and proliferation of RAJI CELLs were then evaluated with florescent (10μl Ethidium-Bromide and Acridine-Orange) staining. Data analyzed by SPSS software (Dunnet and Analysis of variance tests). Findings: The RAJI CELLs showed different responses to different amount of Zinc. Zn had no effects on CELL line up to 100μM at different incubation time points when compared to the control. At higher concentrations (200-500μM), viability diminished significantly at 12 and 24hrs of incubation times when compared to the control (p<0.05). Conclusion: We conclude that Zn has dose-dependent cytotoxic effects on RAJI CELL line and probably it could be used for immune-modulation.    

Background and purpose: Zinc has important effects on human health, specially on structural and functional activities of immune system. This study was carried out to examine the in vitro cytotoxic effects of Zinc on RAJI and Molt-4CELL line.Materials and methods: In this study (by CELL culture) the CELL line was exposed to different concentration of Zinc followed by incubation (37 c, 5% Co2) at various time points (12 to 72 hrs), Viability and proliferation of RAJI and Molt-4 CELLs were then evaluated with florecent (Ethidium-Bromide and Acridine-Orange) staining. Data were analysed using SPSS (Dunet and analysis of varians test).Results: The results showed different responces to different amount of Zinc by The RAJI & Molt-4 CELLs. Concentrations up to 100µM at different incubation time points had no effects on CELL line when compared with the controls. With higher concentrations of Zinc (200-500 µM) viability diminished significantly at 12, 24, ... hrs of incubation times when compared with the controls (p<0.05).Conclusion: We conclude that Zn has dose-dependent cytotoxic effect on RAJI and Molt-4 CELLs.

Background: Zinc has important effects on the human health, especially on the structural and functional activities of immune system. This study was carried out to examine the in vitro cytotoxic effects of Zinc on the RAJI CELL line.Materials and methods: The CELL line was exposed to different concentrations of Zinc followed by incubation (37°C, 5% Co2) at various time points (12 to 72 hrs).Viability and proliferation of RAJI CELLs were then evaluated with florescent (10ml Ethidium-Bromide and Acridine-Orange) staining. Data were analyzed by SPSS (Dunnet and Variance Analysis tests).Results: There was not a significant difference in the response of the RAJI CELLs to different amount of zinc up to 100 mM at different incubation time points. At higher concentrations (200-500 mM) of Zinc, viability diminished significantly at 12, 24 and more hrs of incubation time when compared to the controls (p<0.05).Conclusion: We conclude that Zn has dose-dependent cytotoxic effects on RAJI CELLs and probably could be used for immune-modulation.

Haplophyllum tuberculatum (Forssk. ) A. Juss., is a perennial plant from Rutaceae which is a valuable folk medicinal plant. The leaves, stem and root samples of shadow dried plants gathered from Hormozgan province-IRAN, were extracted with methanol and total phenolics, flavonoids and alkaloids contents were determined by spectrophotometer. Then the methanolic extract was fractionated by di-ethyl-ether, chloroform, butanol, and water respectively. The cytotoxic effects of fractionated extracts were evaluated against RAJI and A549 cancerous CELL lines and intact blood lymphocyte CELLs by MTT assay. Results showed that the most phenolics were accumulated in stems and the most alkaloids and flavonoids contents were observed in the plant leaves. Di-ethyl-ether extract from all three plant organs had moderate cytotoxic effect (IC50 < 200 μ g/mL) against A549 CELL line. Overall cytotoxic effects against A549 were more than RAJI CELL line. None of the extracts had cytotoxicity against intact blood lymphocyte CELLs. It seems that the plant chemicals while have no cytotoxic effects on intact CELLs, have specific effects on different cancerous CELL lines. The results suggest that the purified chemicals of the plant may be benefit for pharmaceutical uses. The present study is the first report on evaluation of cytotoxic activities of fractions with different polarities obtained from methanolic extract of Iranian H. tuberculatum species against A549 and RAJI CELL lines.

Objective: Verotoxin is a member of Shiga toxin family. This family contains AB protein toxins with an enzymatic (A) and a binding (B) compartment. CELLs that have receptor (Gb3) are sensitive to cytotoxic effects of toxin. It has been shown that various tumor CELLs have Gb3 receptor and are selectively sensitive to apoptotic effect of verotoxin. Studies on tumor CELL lines and laboratory animals have shown antineoplastic and antiangiogenesis effects of this toxin. The aim of this study was comparison of cytotoxic effect of verotoxin 1 on two CELL lines: Vero (gold standard for evaluation of cytotoxic effect of Verotoxin) and RAJI (a CELL of a cultured line of lymphoblastoid CELLs derived from a Human Burkitt's lymphoma patients). Materials and Methods: The toxigenic strain was cultured and the production of toxin was evaluated by reverse passive latex agglutination test. Verotoxin 1 was purified by affinity chromatography. Vero and RAJI CELL were treated with serial dilutions of toxin, and viability was evaluated by MTT test. Results: Our result indicated that Verotoxin has cytotoxic effect on RAJI CELL lines. This effect is directly related to toxin concentration. Differences on cytotoxicity of toxin on RAJI CELLs at 1:4-1:128 dilutions in relation to cytotoxicity of toxin on Vero CELLs at the same dilutions were considered statistically significant (P<0.05). But difference of cytotoxicity of toxin at higher dilutions was not significant. Conclusion: Our results revealed that Verotoxin has cytotoxicity on Vero and RAJI CELLs, and this effect on Vero CELLs is more than RAJI CELLs (P<0.05).

Introduction: Zinc has important effects on structural and functional activities of many proteins and enzymes, especially in regulation of immune system. Objective: This study was carried out to examine the in vitro effects of different concentrations of Zinc on viability and morphology of T-Lymphoid (Molt-4) and B-Lymphoid (RAJI) CELL line. Materials and Methods: In this study, the CELL line was exposed to different concentrations of Zinc (100 µM to 500 µM) followed by incubation (37°C, 5%Co2) at various time points (12 to 72 hrs). The CELLs were then evaluated with trypan blue exclusion dye ، and Wright-Gimsa staining.Results: The results of this study showed almost different responses to different amounts of Zinc by the T and B CELL line. Concentrations less than 100 µM of Zinc at different incubation time points had little to no effects on CELL line when compared to the controls. Higher concentrations of Zinc (>100 µM) diminished CELL viability to 70% at 12 hrs and less than 50% at 24 to 72 hrs of incubation times.Conclusion: We concluded that Zinc shows cytotoxic effects (RAJI & Molt-4) depending on the concentrations used. At higher concentrations, it exerts cytotoxic effects.