Background: Physical activity (PA) and Health-related quality of life (HRQoL) are significantly affected in pregnancy. Objectives: Theaimof this studywasto assess these constructsamongNigerian pregnantwomenandalso examine the relationship between them. Methods: A total of 140 pregnant women were recruited from four selected antenatal clinics in Ile-Ife, Osun State, Nigeria, who participated in this cross-sectional study. The pregnancy Physical activity questionnaire (PPAQ); consisting of 33 questions aimed at evaluating different day to day activities and SF-12 Health survey; an eight scale grouped questionnaire were used to assess PA and HRQoL, respectively. Descriptive statistics (mean, standard deviation, frequency, and percentage) and inferential statistics (Pearson product moment correlation and one-way ANOVA) were used for data analysis. Alpha level was set at P < 0. 05. Results: The mean age of the participants was 30. 4 4. 33 years. Household (347. 0 452. 1) and vigorous intensity (14. 3 30. 8) PA constituted the highest and least forms of PA. On the other hand, role limitation-Physical (82. 0 18. 3) and Health perception (82. 0 18. 0), and role limitation-mental (54. 2 50), respectively were the highest and least rated scales on SF-12. The HRQoL scores were not significantly different (P > 0. 05) across the three trimesters of pregnancy except for Physical functioning (P = 0. 023). However, there were significant differences in PA across the three trimester of pregnancy (P < 0. 05), except for vigorous intensity (P = 0. 072), sport (P = 0. 057), and occupational activity (P = 0. 057). There were significant correlations among HRQoL sub-scales and each of household, occupational, and moderate intensity PA only (P < 0. 05). Conclusions: Light to moderate intensity and household PA are predominant among Nigerian pregnant women than vigorous intensity, occupational, and sports-related PA. Higher Health perception and Physical role limitation in HRQoL were mostly demonstrated by the pregnant women. There was a significant inverse relationship between HRQoL and each of moderate intensity, occupational, and household PA.