An experiment was conducted using 360 male chicks for 42 days, and the effect of Protein and PROTEXIN levels on broiler Performance were studied. The experiment was done in a completely randomized design with 2 x 3 factorial arrangements with two Protein levels (NRC and 90% of NRC) and three levels of PROTEXIN (without PROTEXIN, recommended level and 120% of recommended level).  All of the rations were Isocaloric except for Protein and were consisted of NRC recommended levels of nutrients. The results indicated that, PROTEXIN significantly increased weight gain of chicks in growing as well as the whole Production period. (0-42 days) (P<0.05). Feed conversion ratio of chicks fed with 120% of recommended PROTEXIN level was significantly better than the control group in the whole Period (P<0.05). PROTEXIN had no significant effect on feed consumption, carcass Percent, abdominal fat Percent and feed consumption cost. Feed cost decreased with decreasing Protein levels (P<0.05). 120% recommended level of PROTEXIN reduced feed cost Per Kg of live body weight gain, significantly (P<0.05).

The effects of PROTEXIN (probiotic) and formic acid were studied on performance in 200 Cobb broiler chicks in a completely randomized design with 4 treatments and 5 replications and 10 chicks per replicate. The chicks in first group as control were fed with basal diet. The chicks in the second, third and forth groups were fed with food, which contained 0.1 gr/kg PROTEXIN, 0.8 percent formic acid and 0.1 gr/kg PROTEXIN with 0.8 percent Formic acid in diet, respectively. To measurement of performance, broilers and remainder food in each replication were weight, weekly and feed conversion rate were calculated in each week. The results indicated that body weight gain not affected by experimental treatments (P>0.05). PROTEXIN and PROTEXIN + formic acid in grower period and PROTEXIN in finisher period reduced feed intake (P<0.05). Treatment groups improved feed conversion rate in grower and finisher periods (P<0.05) but the forth group were not significantly different from second and third groups (P>0.05). Thymus and bursa of Fabricius weights were increased in treatment groups (P<0.05). It is concluded that PROTEXIN and formic acid improved feed conversion rate and increased weights of thymus and bursa of Fabricius, synergism effect of PROTEXIN and formic acid on performance did not observe.

The aim of this study was to investigate the effect of using the PROTEXIN enzyme (percentage and repetition) as a probiotic agent on the properties of stored bagasse for pulp and paper production. For this purpose, the PROTEXIN was added at three concentrations of %0. 125, %0. 25 and %0. 5 based on oven-dry weight of bagasse and two treatment times: once (adding protoxin at the beginning and storage for six months) and twice (adding PROTEXIN at the beginning and after three months). Then, the treatment bagasse was evaluated for physical properties (weight loss and pH), and chemical compositions. After that, soda pulp was done under active alkali 17%, cooking temperature of 170 ° C and cooking time of 30 minutes. Finally, the properties of pulp and paper were measured and compared with green and control samples without treatment. Results showed that using of PROTEXIN enzyme with a concentration of 0. 5%, twice resulted in the highest conservation status of stored bagasse in terms of the quality of the raw material and pulp and paper properties. In general, the greater the concentration and frequency of using of the enzyme PROTEXIN in bagasse storage, a greater improvement in the quality of bagasse and pulp and paper properties can be achieved.

The effects of probiotics and antibiotics and fat type in diet on broiler performance were studied by using 900 one-day old Ross 308 chicks in a three×three factorial arrangement with three types of fat (nonfat, three percent fatty acid and three percent soy oil) and three growth promoters (without GP, flavomycin and PROTEXIN) in completely randomized design with four replicates per each treatment. Fat and its type had significant effects on weight gain and the body weight gain of birds fed diets containing soy oil were better (P<0.05). Dietary flavomycin supplementation improved weight gain and FCR (P<0.05). The fat type×growth promoter's interaction influenced broiler performance, significantly (P<0.05). Birds fed on diets containing soy oil and flavomycin showed the highest feed intake and weight gain, whereas, the lowest feed intake and weight gain was belonged to birds fed diets containing fat and PROTEXIN (P<0.05). From the results obtained, it can be indicated that addition of PROTEXIN to diets containing fat has negative effect on broiler growth performance.

Effect of PROTEXIN to diminish negative consequences of feeding aflatoxin B1 (AFB1) contaminated diet on performance, immune response, meat quality and ileal microbial flora were examined using 320 sevenday-old Japanese quails fed four treatments [control diet (without additives), diet containing 2.5 mg AFB1/kg, diet containing 150 mg PROTEXIN/kg, diet containing 2.5 mg AFB1/kg+150 mg PROTEXIN/kg] with four replicates and 20 birds each in a completely randomized design. Feed intake of birds fed probiotic diet was higher than those birds in control group (P<0.05). Weight gain in birds fed aflatoxin contaminated diet was lower than birds in other treatments (P<0.05). Humoral immunity response in quails related to aflatoxin and probiotic groups was lower and higher than control group, respectively (P<0.05). Skin thickness increase after 48h challenging with dinitrochlorobenzene (DNCB) in birds fed aflatoxin contaminated diet was lower than the other groups (P<0.05). Concentration of malondialdehyde (after freezing meat for 30 days) in the meat of aflatoxin fed birds was higher than the other birds (P<0.05). Population of E. coli and lactic acid bacteria in birds fed PROTEXIN or aflatoxin+PROTEXIN was lower and higher than the two other groups, respectively (P<0.05). Based on the result of this research, dietary inclusion of PROTEXIN probiotics into aflatoxin contaminated diet could improve immunity response and intestinal microbial population in Japanese quails.

Probiotics are microorganisms which added to the diet in order to improve the health of the host. In the present study, the effects of probiotic, PROTEXIN® on growth, survive and hematological parameters of Benii, Mesopotamichthys sharpeyi (15. 62± 0. 45g) were investigated in Fish Propagation and Rearing Center, Islamic Azad University, Ahvaz. To this aim, 360 fish were assigned to 12 experimental tanks as 3 experimental treatments including T1: 0. 5% Protoxin® , T2: 1% Protoxin® and T3: 1. 5% Protoxin® (triplicate) for 60 days. Also, one group fed with Protoxin-free diet was considered as control. The results of the present study showed that probiotic containing diets especially those fed with 1. 5% Protoxin® (T3) increases weight, specific growth rate, and feed and protein efficiency and also decreases feed conversion ratio. Survival rate values showed no significant differences between experimental groups. The hematological parameters including hemoglobin concentration, the number of monocytes, heterophils and basophils were higher in experimental treatments than control. In contrast, the numbers of red blood and with blood cells were lower in probiotic received groups compared to control group. The Results showed that Protoxin® (especially T3) improve weight index, increase weight, specific growth rate (SGR), protein efficiency, feed efficiency and condition factor but decrease feed conversion ratio (FCR) of Bunni. In conclusion, results suggest that Protoxin® improve growth of Benni.

Effects of probiotic PROTEXIN at 4 doses of 0.15, 0.5,1 and 1.5 g/ kg food on the survival and growth of rainbow trout larvae was investigated, using diet with no protoxin as control. Each treatment tray, with dimensions of 42.5x42.5 cm, was stocked with 265 trout larvae of 90±0.2mg average weight. The experiment was carried out in four replicates. Feeding percentage was ranged between 4%-7% of larval body weight.After 60 days feeding trial, it was found that larvae fed with 0.15g PROTEXIN/kg food had better growth performance than control (P<0.05). Also survival in larvae fed with 0.15g and 1g PROTEXIN /kg food was significantly higher than the with control (P<0.05).