POSITIVE AND NEGATIVE SPACES in the timing of the mosques not only create equilibrium AND balance, but also contain spiritual meanings. But, during different eras, due to influences of external effects, manifested this noble concept, has been declining.In this paper, the POSITIVE AND NEGATIVE SPACES based on mystical AND philosophical concepts, in the tiling frames in the mosque of Imam Khomeini (RA) in Isfahan, the Safavid period AND the mosques AND school of martyr Motahhari as an evidence of Qajar architecture, has been studied. In addition to investigation AND analysis of the POSITIVE AND NEGATIVE SPACES of the tiling of the ‘’Shahid Morahhari’’ school, decorations of this school also has been compared with ‘’Imam Khomeini’’ School as a well-known structure remained from Safavid dynasty that by this way, determine the rate of Changes in the transcendental concepts of visual symbols which were affected by Western art. So that by changes in the fundamental symbols of this type of tile work of art, also dramatically their concepts change AND become transformed. Using a valid index to convey the deep concepts in the foundations of decorating items to present era is so important. According to the investigations, using fundamental items like POSITIVE AND NEGATIVE SPACES in this school show how consistent they are with values AND deep AND conceptual criteria of the far past AND how much they have changed.Research method in this paper is descriptive analysis AND utilizes by comparison system in the idiom analysis.