Background: Green tea (Camellia sinensis) extract is rich source of natural antioxidants specially catechin that is quickly absorbed into the body and it has cancer protective, anti microbial and anti inflammation effects. In this study has been studied role of green tea extract against genotoxic damage induced by cell phone microwaves on bone marrow POLYCHROMATIC ERYTHROCYTEs of adult male Balb/C mouse.Materials and Methods: In this experimental study 40 mouse were divided into five groups, control animals were located under natural condition, sham -exposed animals were prepared by experimental condition without cell phone waves radiation. Experimental 1 group that irradiated at cell phones for 4 days (3 hours/day) and experimental 2 groups were injected intraperitoneal 100 mg/kg green tea extract for 5 days and experimental 3 group that irradiated at active mobile phones for 4 days (3 hours/day) and were injected intraperitoneal 100 mg/kg green tea extract for 5 days. After treatment period micronucleus test was evaluated in POLYCHROMATIC ERYTHROCYTEs on bone marrow. The quantitative data was analyzed by ANOVA and Tukey test with using of SPSS-13 software at the level of p<0.05.Results: Based on this study, treatment with extracts of green tea decreased micronucleus frequency in bone marrow POLYCHROMATIC ERYTHROCYTEs of Balb/C mouse that irradiated at cell phone microwave (0.92±0.129), (p<0.001).Conclusion: Cell phone microwaves (940 MHz) increased micronucleus on bone marrow POLYCHROMATIC ERYTHROCYTEs of male Balb/C mouse, but green tea had inhibitory effect and it decreased the average number of micronucleus.

Objective: Ionizing radiation produces free radicals which induce DNA damage and cell death. Origanum vulgare leaf extract (OVLE) is a natural compound and its capability of scavenging free radicals and its antioxidant activity have been demonstrated by many researchers. In this study, using micronucleus assay, radioprotective effect of OVLE against clastogenic and cytotoxic effect of gamma irradiation has been investigated in mice bone marrow cells.Materials and Methods: OVLE was injected intraperitoneally to the BALB/c mice 1hr prior to gamma irradiation (3Gy) at the doses of 100 and 200 mg/kg. Twenty four hours after irradiation or treatment, animals were killed and smears were prepared from the bone marrow cells. The slides were stained with May Grunwald–Giemsa method and analyzed microscopically. The frequency of micronucleated POLYCHROMATIC ERYTHROCYTEs (MnPCEs), micronucleated normochromatic ERYTHROCYTE (MnNCEs) and cell proliferation ratio PCE/PCE+NCE (POLYCHROMATIC ERYTHROCYTE/POLYCHROMATIC ERYTHROCYTE+normochromatic ERYTHROCYTE) were calculated.Results: The results showed that gamma irradiation (3Gy) increased the frequency of MnPCEs, MnNCEs and reduced the PCE/PCE+NCE ratio in mice bone marrow compared to the non-irradiated control group (p<0.0001). Injection of OVLE significantly reduced the frequency of MnPCEs (p<0.0001) and MnNCEs (p<0.05) and increased the PCE/PCE+NCE ratio as compared to the irradiated control group (p<0.05).Conclusion: It seems that OVLE with its antioxidant properties and its capability of scavenging free radicals and reactive oxygen species can reduce the cytotoxic effects of gamma irradiation in mice bone marrow cells.

Background: Ionizing radia on irradiated from iodine-131 can induce DNA damage and cell death. The cellular DNA damage is the cause of muta on and cancer. The micronucleus assay in polychroma c ERYTHROCYTEs was applied to assess the radio-protec ve effect ofTurmeric extract on genotoxic poten al of iodine therapy.Materials and Methods: Thirty six male albino rats were randomly divided in six groups. A single dose (200 or 500 mg/kg) ofTurmeric extract was injected to the rats 30 min before iodine therapy. Iodine-131 (5.55 MBq) was administrated intra peritoneal to the experimental animals.The percentage of micronuclei in PCE, NCE and ra o of PCE / (PCE+NCE) was determined 48 h a6er iodine injec on for each experimental group to assess iodine-131 radia on effects with or withoutTurmeric extract. Results: Iodine therapy showed a significant increase in the number of micronucleus forma on. The animals treated with different doses ofTurmeric extract+ iodine showed a significant reduc on in the frequency of micronucleus compared to the animals treated with iodine-131 alone. Both doses of Turmericextract had the same effect when injected 30 min prior to iodine therapy.Conclusion: Our results indicate protec ve effect of Turmeric extract against gene c damages induced by iodine-131 administra on.

Background: There is unprecedented increase in the processing and packaging of many plant materials into food supplements, herbal medicine, skincare and cosmetic products for human needs.Baphia nitida is used for topical skincare products. Toluene, a toxic aromatic solvent, is increasingly being used in the production of these skincare and cosmetic products in many industries. This study assessed toluene toxicological profile and the ability of Baphia nitidadye to ameliorate toluene induced cytogenotoxicity, hematotoxicity and histopathological effects in rats.Methods: Rats were treated with various concentrations; 0, 1000, 2000 and 5000 mg of the aqueous, ethanol and toluene processed B. nitida dye via dermal exposure for acute and sublethal toxicity.96 h acute toxicity was assessed for the solvents. Micronuclei induction, alterations in hematological indices and ERYTHROCYTE morphology and skin histology were assessed after sub-lethal treatment.Results: 96 h LD50 of B. nitida processed dye for the three solvents were indeterminate. There was insignificant (p>0.05) alterations in the hematological indices and ERYTHROCYTE morphology, induction of micronucleated POLYCHROMATIC ERYTHROCYTE and POLYCHROMATIC and normochromatic ERYTHROCYTE ratio in the aqueous and ethanol processed B. nitida treated rats compared to their corresponding controls. Toluene induced significant (p<0.05) decrease in ERYTHROCYTEs count, hematocrit and leucocytes, increased micronucleated PCE, decreased PCE/NCE ratio and induced necrosis, thick dermal layer and dispersed areolar tissues in treated rats. But, these effects were ameliorated by the B. nitida dye.Conclusion: Camwood dye protects against toluene induced toxicity in rats. This suggests its relative safety in topical cosmetic and skincare production.

Introduction: To study the effects of Polarized POLYCHROMATIC Noncoherent Light (Bioptron) therapy on patients with carpal tunnel syndrome (CTS).Methods: This study was designed as a randomized clinical trial. Forty four patients with mild or moderate CTS (confirmed by clinical and electrodiagnostic studies) were assigned randomly into two groups (intervention and control goups). At the beginning of the study, both groups received wrist splinting for 8 weeks. Bioptron light was applied for the intervention group (eight sessions, for 3/weeks). Bioptron was applied perpendicularly to the wrist from a 10 centimeters distance. Pain severity and electrodiagnostic measurements were compared from before to 8 weeks after initiating each treatment.Results: Eight weeks after starting the treatments, the mean of pain severity based on Visual Analogue Scale (VAS) scores decreased significantly in both groups. Median Sensory Nerve Action Potential (SNAP) latency decreased significantly in both groups. However, other electrophysiological findings (median Compound Motor Action Potential (CMAP) latency and amplitude, also SNAP amplitude) did not change after the therapy in both groups. There was no meaningful difference between two groups regarding the changes in the pain severity.Conclusion: Bioptron with the above mentioned parameters led to therapeutic effects equal to splinting alone in patients with carpal tunnel syndrome. However, applying Bioptron with different therapeutic protocols and light parameters other than used in this study, perhaps longer duration of therapy and long term assessment may reveal different results favoring Bioptron therapy.

Dear Editor, ERYTHROCYTE sedimentation rate (ESR) is a useful basic clinical pathology laboratory investigation. It can be helpful in diagnosis and follow-up of several diseases. At present, a new automated method with proven reliability is available for ESR test (1). Here, the authors report on observation on a laboratory experiment to test the effect of zinc nanoparticles on ESR results. The total of 100 blood samples was used in the experiment. Each sample was divided into two parts. One part was directly measured for ESR and the other part was added by 1 droplet of zinc nanoparticles solution then exposed to ESR measurement.