In order to investigate the effect of biochar and biological fertilizer on PHOTOSYNTHETIC PIGMENTS, yield, and nutrient content of peppermint (Mentha piperita L. ) an experiment has been conducted as factorial, based on a randomized complete block design in three replications at Sari Agricultural Sciences and Natural Resources University in 2017. The treatments include biochar on two levels (0 and 10 ton ha-1 organic and biological fertilizers on five levels (no fertilizer (F0), vermicompost (10 ton ha ) and -1 ) (F1), Pseudomonas fluorescens (F2), Azotobacter (F3), and Arbuscular mycorrhiza (Glomus mosseae, G. intradicces, and G. etunicatum) (F4)). Results from this study show that the use of biochar increases the chlorophyll a and chlorophyll content by 7. 42% and 9. 71%, respectively. Among fertilizer treatments, the highest amount of chlorophyll belongs to vermicompost (6. 11 μ g. ml-1 ), significantly better than other treatments. In addition, the main effect of biochar and bio-fertilizer have had a significant effect on leaf, stem, and total fresh weight of the plant. Results from comparing mean of fertilizer treatments show that the highest leaf fresh weight, stem fresh weight, and total fresh weight per square meter belong to the treatment of mycorrhiza (306. 08, 361. 15, and 663. 03 g. m-2 respectively). Also, the use of biochar increases the leaf fresh weight, stem fresh weight, and total fresh weight (294. 78, 366. 73, and 661. 46 g. m-2 respectively), compared to the conditions without using it. Based on the results of the comparison of the mean of the main effects, all treatments increase nitrogen content, compared to the control. The highest nitrogen content (1. 83%) is related to Azotobacter treatment, not significantly different from vermicompost, mycorrhiza, and Pseudomonas. Results also suggest that the maximum amount of phosphorus (0. 37%) have been seen in biochar + mycorrhiz and biochar + vermicompost. Therefore, with respect to the production of medicinal plants in the low-input cropping systems, it is recommended to protect the environmental and achieve sustainable agriculture to improve plant growth and photosynthesis of peppermint application of biochar + mycorrhiz along with biochar + vermicompost treatment.